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Dictionary » B » Blot western Blot westerna technique in molecular biology, used to separate and identify proteins. Called a western blot merely because it has some similarity to a southern blot (which is named after its inventor, the British biologist M.E. Southern).
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Results from our forumIn Vitro Transcription Translation... and autoradiographed to visulalize.. Do i have to also prove by western blot ? There are no background lysate proteins shown up in gel. Its pretty good. Any advice. ...
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TCA preipitation... of supernatant concentrated down to 100uL to detect the protein on a western blot)..How do I get 50mL of supernant? Please help. I'm so confused :cry: Thanks!! b_06er
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Protein isolationHow can you isolate any protein with Western blot? :roll: Trizol should work for protein extraction, but I have never used it. I guess they will be ...
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Protein isolation... protein extraction, and HIF 1 alpha seems to be favorably isolated with western blot. If anyone has any input on this, I would greatly appreciate it. Thank you.
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Western Blot... I have a question, how can we know about the size of protein in the western blot? I know the full lenght, but after WB, I am seeing band with a different size! Thanks ...
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