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Blot western

Blot, western

a technique in molecular biology, used to separate and identify proteins. Called a western blot merely because it has some similarity to a southern blot (which is named after its inventor, the British biologist M.E. Southern).


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mRNA vs. cell reporter assay

... Maybe the reporter system provides us with more information than RT-PCR/Western blot, because it proves that the transcription factor is actually active too, not just present? ...

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by kk
Mon Sep 08, 2014 2:57 pm
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 458

Re: Western transfer failure

... in transfer buffer. I was transferring two 12% SDS-PAGE gels to PVDF in one tank. 100V, 1 hr. One gel transferred fine, another went backwards to blot paper after 1 hr. Did I just arrange backwards? Did lack of SDS mess with electric gradient? Correlation may not be causation, so I don't claim ...

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by yanggers
Thu Feb 06, 2014 9:16 pm
 
Forum: Molecular Biology
Topic: Western transfer failure
Replies: 5
Views: 5124

Weird - shaped band on western blotting

... cell cycle regulation protein. Recently I got a weird-shaped band on my western blot. It is like an upside-down of smiling face in each lane. I use the same loading buffer, ...

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by namakulaila
Thu Oct 17, 2013 6:17 am
 
Forum: Molecular Biology
Topic: Weird - shaped band on western blotting
Replies: 2
Views: 1401

In Vitro Transcription Translation

... and autoradiographed to visulalize.. Do i have to also prove by western blot ? There are no background lysate proteins shown up in gel. Its pretty good. Any advice. ...

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by Shaanu
Mon Jun 03, 2013 7:12 am
 
Forum: Molecular Biology
Topic: In Vitro Transcription Translation
Replies: 3
Views: 1994

TCA preipitation

... of supernatant concentrated down to 100uL to detect the protein on a western blot)..How do I get 50mL of supernant? Please help. I'm so confused :cry: Thanks!! b_06er

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by biology_06er
Wed Feb 27, 2013 3:25 am
 
Forum: General Discussion
Topic: TCA preipitation
Replies: 2
Views: 2422
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