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Blot southern

Blot, southern

a common test for checking for a match between dna molecules. Dna fragments are separated by agarose gel electrophoresis, transferred (blotted) onto membrane filters, and hybridised with complementary radiolabelled probes. The aim is to detect specific base sequenceswith the probes. Lest all of this sound esoteric, note that in the television series The X files a southern blot was done (in this case, to learn if some alien virus genome had been integrated into a persons genome). The southern blot is named after its inventor, the British biologist M.E. Southern. There is also a northern blot and a western blot.


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Souther blot and restriction mapping

... greatly appreciate any help with constructing a restriction map from Southern Hybridisation data. I can construct restriction maps from fragment ... and separation by standard gel electrophoresis, however, Southern Blot data only shows those fragments that a probe has hybridised to and I ...

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by Mistersnuffle
Sat Oct 04, 2014 11:17 am
 
Forum: Genetics
Topic: Souther blot and restriction mapping
Replies: 0
Views: 25

molecular biology

... The following gel diagram shows the pattern of bands observed in a Southern blot for three tightly linked SSR molecular markers in and flanking the p16 gene in a family ...

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by soniasunny111
Sat Aug 20, 2011 10:45 am
 
Forum: General Discussion
Topic: molecular biology
Replies: 1
Views: 2547

finding out if resistance is from mutation or hybridization?

... gives the resistance), that isn't in the wildtype. Could a Southern blot be used to test for this microsat? If so, how would we know if it was weedy rices own ...

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by AE35
Sat Jun 11, 2011 12:48 am
 
Forum: Genetics
Topic: finding out if resistance is from mutation or hybridization?
Replies: 4
Views: 2335

nuclear acid probe labeling

... manual that is available was generally a very good information for all Southern and Northern blot related thing (even if you use other products)

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by canalon
Sat Sep 25, 2010 6:16 pm
 
Forum: Molecular Biology
Topic: nuclear acid probe labeling
Replies: 3
Views: 2594

Re:

... cases. Okay So I could just cut one sequence with ECO R1 and then do a southern blot hybridization. I understand the concept of cutting. But I don't understand the concept ...

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by doctobe
Sat Feb 20, 2010 6:31 am
 
Forum: Molecular Biology
Topic: DNA sequenced. Erased labels. Now re-identify.
Replies: 12
Views: 4008
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