Dictionary » B » Blot

Blot

Blot

1. To spot, stain, or bespatter, as with ink. The brief was writ and blotted all with gore. (Gascoigne)

2. To impair; to damage; to mar; to soil. It blots thy beauty, as frosts do bite the meads. (Shak)

3. To stain with infamy; to disgrace. Blot not thy innocence with guiltless blood. (Rowe)

4. To obliterate, as writing with ink; to cancel; to efface; generally with out; as, to blot out a word or a sentence. Often figuratively; as, to blot out offenses. One act like this blots out a thousand crimes. (Dryden)

5. To obscure; to eclipse; to shadow. He sung how earth blots the moons gilded wane. (Cowley)

6. To dry, as writing, with blotting paper.

Synonym: to obliterate, expunge, erase, efface, cancel, tarnish, disgrace, blur, sully, smear, smutch.

Origin: cf. Dan. Plette. See blot.


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Re: Western transfer failure

... in transfer buffer. I was transferring two 12% SDS-PAGE gels to PVDF in one tank. 100V, 1 hr. One gel transferred fine, another went backwards to blot paper after 1 hr. Did I just arrange backwards? Did lack of SDS mess with electric gradient? Correlation may not be causation, so I don't claim ...

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by yanggers
Thu Feb 06, 2014 9:16 pm
 
Forum: Molecular Biology
Topic: Western transfer failure
Replies: 5
Views: 4005

Re: qPCR vs Northern Blot

Thank you both for answers. Firstly - aliquots for Northern blot and qPCR were the same (firstly I did NB and after a couple of weeks qPCR). @jonmoulton - you're rigth first - in Northern blot I had silencing, in qPCR i didn't. As far as your question ...

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by Nevthonien
Tue Nov 12, 2013 2:47 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 1274

Re: qPCR vs Northern Blot

I see that I reversed the sense of the observations - the Northern signal was maintained, the PRC signal decreased. A splicing change could still produce that result, as could the RNA degradation Jack mentioned.

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by jonmoulton
Mon Nov 11, 2013 4:38 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 1274

electrophoretic mobility shift assay (EMSA)

... after the first try having success, I could never reproduce my results: following the streptavidin-biotin-HRP detection, there are no bands on the blot paper. Compared to the first succesful try, the most striking difference I experience is that while at first, there was a bromophenol blue front ...

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by kk
Fri Nov 08, 2013 12:21 pm
 
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 648

Re: qPCR vs Northern Blot

I assume you are assaying aliquots of the same RNA samples by the two methods. Does your Northern probe bind between the binding sites of your PCR primers? If not, it is conceivable that a change in splicing in the presence of the shRNA causes your Northern target to be spliced out of the pre-mRNA w...

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by jonmoulton
Thu Nov 07, 2013 10:12 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 1274
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