Dictionary » B » Blot

Blot

Blot

1. To spot, stain, or bespatter, as with ink. The brief was writ and blotted all with gore. (Gascoigne)

2. To impair; to damage; to mar; to soil. It blots thy beauty, as frosts do bite the meads. (Shak)

3. To stain with infamy; to disgrace. Blot not thy innocence with guiltless blood. (Rowe)

4. To obliterate, as writing with ink; to cancel; to efface; generally with out; as, to blot out a word or a sentence. Often figuratively; as, to blot out offenses. One act like this blots out a thousand crimes. (Dryden)

5. To obscure; to eclipse; to shadow. He sung how earth blots the moons gilded wane. (Cowley)

6. To dry, as writing, with blotting paper.

Synonym: to obliterate, expunge, erase, efface, cancel, tarnish, disgrace, blur, sully, smear, smutch.

Origin: cf. Dan. Plette. See blot.


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Souther blot and restriction mapping

... restriction maps from fragment data obtained through endonuclease digestion and separation by standard gel electrophoresis, however, Southern Blot data only shows those fragments that a probe has hybridised to and I am struggling to find the correct approach. The parting words from lecturer ...

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by Mistersnuffle
Sat Oct 04, 2014 11:17 am
 
Forum: Genetics
Topic: Souther blot and restriction mapping
Replies: 0
Views: 22

mRNA vs. cell reporter assay

I have the same question, phrased in a slightly different way: Maybe the reporter system provides us with more information than RT-PCR/Western blot, because it proves that the transcription factor is actually active too, not just present? I thought it is enough to detect a significant increase ...

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by kk
Mon Sep 08, 2014 2:57 pm
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 694

DNA testing times

... done in a working day, though the PCR thermal-cycling machine can be set up and left to run overnight. For most DNA methods, whether PCR or blot, an hour is much too short. The time required for analysis will depend on how much information the assay provides and how complex a question you ...

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by jonmoulton
Fri Apr 11, 2014 2:47 pm
 
Forum: Genetics
Topic: DNA testing times
Replies: 2
Views: 1211

Re: Western transfer failure

... in transfer buffer. I was transferring two 12% SDS-PAGE gels to PVDF in one tank. 100V, 1 hr. One gel transferred fine, another went backwards to blot paper after 1 hr. Did I just arrange backwards? Did lack of SDS mess with electric gradient? Correlation may not be causation, so I don't claim ...

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by yanggers
Thu Feb 06, 2014 9:16 pm
 
Forum: Molecular Biology
Topic: Western transfer failure
Replies: 5
Views: 5239

Re: qPCR vs Northern Blot

Thank you both for answers. Firstly - aliquots for Northern blot and qPCR were the same (firstly I did NB and after a couple of weeks qPCR). @jonmoulton - you're rigth first - in Northern blot I had silencing, in qPCR i didn't. As far as your question ...

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by Nevthonien
Tue Nov 12, 2013 2:47 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 2785
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