Blocking antibody

... would preferentially bind to where the lanes are and not in between my antibody is phospho-p38 MAPK kinase(Thr180/Tyr182) antibody. Usually, for this antibody, we used 1hr 5% BSA blocking, and incubation overnigh with primary antibody. In recent months, we used ...

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I did detection again today, using same antibody, but varied protocol. yesterday, i used 5% BSA blocking, and blocked 1hr, then 1st antibody incubation overnight at 4 degree. today, i used 5% skim ...

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... in vivo, and i found a new subcellular location for a protein with one antibody (which has been use in many publication). Pre-incubation with the immunogen peptide (blocking peptide) result in the loss of the signal. Overexpression of this protein in ...

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... of the pathway and see what happens. Eliminate some receptor with an antibody or make a knock-out organism that lacks a gene for, say, some enzyme, ... including PCR, different blottings, monocolonal antibody staining/blocking, knock-out and/or transgenic organisms (cells, bacteria, plants, ...

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... enough about either to offer much advice. Make sure you're diluting the antibody and doing any blocking correctly--but I imagine you've checked over obvious things like that. Maybe try a ...

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