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Blocking antibody

Blocking antibody

An antibody used in a reaction to prevent some other reaction taking place, for example one antibody competing with another for a cell surface receptor.

See: desensitisation.


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Re:

... would preferentially bind to where the lanes are and not in between my antibody is phospho-p38 MAPK kinase(Thr180/Tyr182) antibody. Usually, for this antibody, we used 1hr 5% BSA blocking, and incubation overnigh with primary antibody. In recent months, we used ...

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by jasmina
Sat Apr 10, 2010 5:59 am
 
Forum: Cell Biology
Topic: western results
Replies: 6
Views: 1536

Re: western results

I did detection again today, using same antibody, but varied protocol. yesterday, i used 5% BSA blocking, and blocked 1hr, then 1st antibody incubation overnight at 4 degree. today, i used 5% skim ...

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by jasmina
Fri Apr 09, 2010 6:29 am
 
Forum: Cell Biology
Topic: western results
Replies: 6
Views: 1536

antibody staining

... in vivo, and i found a new subcellular location for a protein with one antibody (which has been use in many publication). Pre-incubation with the immunogen peptide (blocking peptide) result in the loss of the signal. Overexpression of this protein in ...

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by gael
Tue Nov 03, 2009 10:33 am
 
Forum: Cell Biology
Topic: antibody staining
Replies: 2
Views: 1575

Photosynthesis

... of the pathway and see what happens. Eliminate some receptor with an antibody or make a knock-out organism that lacks a gene for, say, some enzyme, ... including PCR, different blottings, monocolonal antibody staining/blocking, knock-out and/or transgenic organisms (cells, bacteria, plants, ...

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by biohazard
Thu Aug 13, 2009 10:42 am
 
Forum: Cell Biology
Topic: Photosynthesis
Replies: 10
Views: 7978

N-Cadherin western blotting

... enough about either to offer much advice. Make sure you're diluting the antibody and doing any blocking correctly--but I imagine you've checked over obvious things like that. Maybe try a ...

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by blcr11
Mon Dec 10, 2007 2:04 pm
 
Forum: Molecular Biology
Topic: N-Cadherin western blotting
Replies: 2
Views: 1396
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