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Blocking activity

Blocking activity

The repression or elimination of electrical activity in the brain by the arrival of a sensory stimulus.


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Re: How do you overexpress and knock down a gene

... not be very specific. The antisense transcript will trigger RNase-H activity and the shRNA will use argonaute activity; both of these enzymatic ... such as siRNA or shRNA. The most specific approach is to deliver steric-blocking antisense oligos, which do not use enzymatic systems like argonaute ...

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by jonmoulton
Tue Nov 30, 2010 10:27 pm
 
Forum: Molecular Biology
Topic: How do you overexpress and knock down a gene
Replies: 2
Views: 13325

Re: is it possible to knockdown a gene in a living adult now

... of steric block morpholino oligomers by (R-X-R)4 peptides: structure-activity studies. Nucleic Acids Res. 2008 Sep 16. [Epub ahead of print] Bok ... conjugates} Moulton HM, Moulton JD. Peptide-assisted delivery of steric-blocking antisense oligomers. Curr Opin Mol Ther. 2003 Apr;5(2):123-32. {review} ...

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by jonmoulton
Fri Nov 07, 2008 3:44 pm
 
Forum: Genetics
Topic: is it possible to knockdown a gene in a living adult now
Replies: 10
Views: 15145

Re: how to knock out a gene?

... 1. Realizing that the arginine was crucial to the peptide's delivery activity and that it was the head-group of arginine, a guanidinium group, ... Moulton HM, Moulton JD. Peptide-assisted delivery of steric-blocking antisense oligomers. Curr Opin Mol Ther. 2003 Apr;5(2):123-32. We ...

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by jonmoulton
Fri Oct 17, 2008 4:38 pm
 
Forum: Genetics
Topic: how to knock out a gene?
Replies: 4
Views: 7674

Re: splicing MO vs translation MO

... methods; I prefer the use of a second non-overlapping translation-blocking oligo. If each of the translation blockers, used in separate experiments, ... confident that insertion of the intron will inactivate the protein's activity. Let me know how I can help. - Jon (from Gene Tools)

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by jonmoulton
Mon Aug 18, 2008 6:19 pm
 
Forum: Molecular Biology
Topic: splicing MO vs translation MO
Replies: 14
Views: 11549

Re: Morpholino review: how to knockdown

... There are many cases where rescues have been reported using translation blocking Morpholino oligos targeting the 5'-UTR of an mRNA. A construct is ... 5'-UTR sequence divergence but can complement the missing protein's activity) or using a Morpholino that targets the start of the coding sequence ...

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by jonmoulton
Thu Jul 31, 2008 9:30 pm
 
Forum: Molecular Biology
Topic: Morpholino review: how to knockdown
Replies: 7
Views: 13536
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