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Biotin

Biotin

(Science: biochemistry) a prosthetic group for carboxylase enzymes. Important in fatty acid biosynthesis and catabolism and has found widespread use as a covalent label for macromolecules which may then be detected by high affinity binding of labelled avidin or streptavidin. Essential growth factor for many cells. A B vitamin that aids in body growth.A vitamin, deficiency symptoms range from dermatitis to intestinal problems.


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electrophoretic mobility shift assay (EMSA)

I am doing EMSA and after the first try having success, I could never reproduce my results: following the streptavidin-biotin-HRP detection, there are no bands on the blot paper. Compared to the first succesful try, the most striking difference I experience is that while at first, ...

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by kk
Fri Nov 08, 2013 12:21 pm
 
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 1218

Biotinylation

one question about biotinylation. After biotinyled with BIOTIN, and then lysated cells in RIPA buffer and then added strepavidin -beads for 4 hours at 4c, RUN SDS-PAGE gel, probed with the antibody I am interested, should I reprobe the blot with tubulin ...

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by piglet1
Thu Oct 07, 2010 7:45 pm
 
Forum: Cell Biology
Topic: Biotinylation
Replies: 5
Views: 2394

Help with the design of an ELISA experiment

... the rabbit anti-mouse IgG. And I'd use goat anti-mouse IgG FC-HRP. Is this at all right? With that what's the difference between ap-conjugate and biotin? And FC-HRP and gamma chain HRP? Which of these do I choose? And what's the purpose of mouse serum albumin and rabbit anti-mouse serum? Sorry ...

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by bemardus
Wed Apr 28, 2010 4:18 am
 
Forum: Molecular Biology
Topic: Help with the design of an ELISA experiment
Replies: 4
Views: 3186

Help with the design of an ELISA experiment

... serum is a result of IgG. What I have to work with is mouse serum, mouse serum albumin, mouse IgG, rabbit anti-mouse IgG, goat anti-mouse IgG (biotin and FC-HRP), goat anti rabbit IgG (biotin and ap-conjugated), goat anti-mouse IgG HRP (gamma chain), rabbit anti mouse serum. I haven't an idea ...

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by bemardus
Tue Apr 27, 2010 10:39 pm
 
Forum: Molecular Biology
Topic: Help with the design of an ELISA experiment
Replies: 4
Views: 3186

The Fiber Disease

... possible physiological roles of metallothionein (MT), we have studied immunohistological localization of MT in the eye of the rat, using an avidin-biotin peroxidase complex method. As a result, strong MT immunostaining was observed in the epithelium of the lens and cornea. In the retina, considerably ...

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by London
Sat Dec 02, 2006 9:51 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5479040
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