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Biotin

Biotin

(Science: biochemistry) a prosthetic group for carboxylase enzymes. Important in fatty acid biosynthesis and catabolism and has found widespread use as a covalent label for macromolecules which may then be detected by high affinity binding of labelled avidin or streptavidin. Essential growth factor for many cells. A B vitamin that aids in body growth.A vitamin, deficiency symptoms range from dermatitis to intestinal problems.


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The Fiber Disease

... possible physiological roles of metallothionein (MT), we have studied immunohistological localization of MT in the eye of the rat, using an avidin-biotin peroxidase complex method. As a result, strong MT immunostaining was observed in the epithelium of the lens and cornea. In the retina, considerably ...

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by London
Sat Dec 02, 2006 9:51 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 712702

The Fiber Disease

... electrophoresis in the other . The function of the device is demonstrated by detecting DNA targets . Single-strand DNA (ssDNA) probes labeled with biotin were immobilized onto microbeads coated with streptavidin . The DNA-functionalized microbeads were packed into each of three microchambers by ...

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by Nadas Moksha
Sat Nov 25, 2006 4:34 am
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 712702

Re: Denaturing agents to convert d.s Lambda DNA to s.s DNA

... 2.What are the denaturing agents that can be used to denature a double strand (e.g. Lambda DNA) to get a single strand, preserving streptavidin-biotin bonds? Also what’s the ideal pH and salt concentration to keep the single strand intact after the double stranded DNA was denatured? Any kind ...

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by shankargan2006
Wed Nov 15, 2006 1:01 am
 
Forum: Molecular Biology
Topic: Re: Denaturing agents to convert d.s Lambda DNA to s.s DNA
Replies: 0
Views: 389

biotin

It is a co-enzyme. And it has a role in CO2 transfer. For more: http://en.wikipedia.org/wiki/Biotin

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by Poison
Thu Sep 07, 2006 11:53 am
 
Forum: Molecular Biology
Topic: biotin
Replies: 1
Views: 602

biotin

anybody knows the function of biotin in gluconeogenesis and lipid synthesis?

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by kabuto
Thu Sep 07, 2006 7:02 am
 
Forum: Molecular Biology
Topic: biotin
Replies: 1
Views: 602
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