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Biological assay

Biological assay

(Science: technique) once a pharmaceutical protein is isolated from the cells in which it was grown, researchers perform tests to measure the proteins biological activity.

It must maintain a certain minimal level of biological activity to be used for animal or clinical testing or, later, for market. Researchers also test to confirm that the isolated protein is identical to the desired protein.


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Re: Gluten-free and Vaccines

... cases, available information suggested that this homology could have biological relevance. For example, the region of pertactin that is homologous ... vivo using the rat intestinal perfusion model (Fig. 4B). In the latter assay, the synergistic effect of PEP and BBM peptidases was evident. Moreover, ...

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by tmerezko
Tue Jun 22, 2010 6:08 pm
 
Forum: General Discussion
Topic: Gluten-free and Vaccines
Replies: 7
Views: 17821

buffer

... told to dilute Tris-HCL buffer from 1M to 10mM before using it for a biological assay. Instead of doing that, he used the 1M solution directly in his assay, citing that a ...

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by jojo90
Sun Apr 27, 2008 3:00 am
 
Forum: General Discussion
Topic: buffer
Replies: 3
Views: 1405

The Fiber Disease

... reproducible, and in vivo-like cell culture, leading to more biologically accurate results. Nanofiber Inserts These inserts provide a ... for uniformity of fiber size distribution and tested in an attachment assay using the PC12 cell line. Cells Cultured on Ultra-Web Synthetic ECM ...

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by RANDY
Thu Jan 18, 2007 3:39 am
 
Forum: Human Biology
Topic: The Fiber Disease
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Views: 5563451

The Fiber Disease

... light is superior to that of the other colors for visualization of biological functions in living cells. Now, different color luciferases, which ... luciferases, Nakajima et al. developed a tricolor reporter in vitro assay system based on these different color luciferases in which the expression ...

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by London
Wed Dec 27, 2006 12:26 am
 
Forum: Human Biology
Topic: The Fiber Disease
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... the 3-(4,5 dimethylthiozol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay, the graft copolymer has been proved to have low cytotoxicity. Based ... drying: Influence of atomization on protein aggregation and biological activity. CHEMTRAILS per condensate anyone????????????? Protein ...

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by Nadas Moksha
Wed Dec 20, 2006 8:01 pm
 
Forum: Human Biology
Topic: The Fiber Disease
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