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Binding sites

Binding sites

The reactive parts of a macromolecule that directly participate in its specific combination with another molecule.


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Re: qPCR vs Northern Blot

... samples by the two methods. Does your Northern probe bind between the binding sites of your PCR primers? If not, it is conceivable that a change in splicing in the presence ...

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by jonmoulton
Thu Nov 07, 2013 10:12 pm
 
Forum: Molecular Biology
Topic: qPCR vs Northern Blot
Replies: 4
Views: 1292

Comprehensive List of TFBSs database from ChIP-Seq data

Comprehensive List of transcription factor binding sites (TFBSs) databases based on ChIP-seq data as follows: (1) ChEA : transcription factor regulation ...

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by rnaworld2110
Fri Feb 15, 2013 6:59 am
 
Forum: Molecular Biology
Topic: Comprehensive List of TFBSs database from ChIP-Seq data
Replies: 0
Views: 1650

Re: PCR

... human (unless the primers are poorly chosen and so there is off-target binding due to allelic variation). So, you prime at the locus of the forward ... is because the distance between the forward and reverse primer binding sites varies between individuals. "What are the most likely explanations ...

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by jonmoulton
Tue Jan 29, 2013 4:16 pm
 
Forum: Genetics
Topic: PCR
Replies: 2
Views: 2177

Re: parallel binding of miRNA with mRNA

... of this miRNA? For your convenience i would like to add that this predicted miRNA doesn't match with any reported miRNA in mirBase or any other sites. Any one can help?

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by tareq07
Sun Jan 20, 2013 8:53 am
 
Forum: Genetics
Topic: parallel binding of miRNA with mRNA
Replies: 9
Views: 3702

Re: what is the purpose of ChIP experiment?

To localize protein binding sites in the DNA. For example sites where transcription factors (proteins, products of other genes) bind. This ...

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by Lucanus cervus
Tue Jun 05, 2012 3:07 am
 
Forum: Molecular Biology
Topic: what is the purpose of ChIP experiment?
Replies: 1
Views: 1389
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