Dictionary » B » Binding


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(Science: biochemistry, chemistry, molecular biology) The adherence of molecules to one another, for example, enzymes to substrates, antibodies to antigens, dna strands to their complementary strands.

binding occurs because the shape and chemical natures of parts of the molecules surfaces are complementary. a common metaphor is the lock-and-key, used to describe how enzymes fit around their substrate.

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Main Factors that Affect Clinical Consequence of Toxicity

... also affects its availability for absorption. Adjuvants are formulation factors used to alter the toxicologic effect of the active ingredient. The binding agents, enteric coating, and sustained-release preparations influence absorption of the active ingredient. As absorption is delayed, toxicity ...

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by 2444256703
Thu Jan 29, 2015 7:30 am
Forum: Molecular Biology
Topic: Main Factors that Affect Clinical Consequence of Toxicity
Replies: 0
Views: 262

western blotting of DNA-binding protein of mammalian cell

i am trying to immunoblot one of the DNA-binding protein.... first i have cultured the cells in 25 cm2 flask and collected the cell lysate using RIPA lysis (1 ml) buffer...kept in ice for 5 min, followed by passing the lysate through 24 G needle ...

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by drelamuruganvet
Mon Jan 19, 2015 9:39 am
Forum: Molecular Biology
Topic: western blotting of DNA-binding protein of mammalian cell
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Views: 394

Re: immunoglobulins

... (Ig) are IgA, IgD, IgE, IgG, and IgM. (See accompanying figure.) Each class varies in its chemical structure and in its number of antigen-binding sites and adheres to and reacts only with the specific antigen for which it was produced.

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by RZachary1
Wed Jan 14, 2015 5:49 am
Forum: Cell Biology
Topic: immunoglobulins
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Views: 2185

I have got some BIG issues! forgive the long post

... approximately 34000 and a lighter chain with a molecular weight of approximately 29000. They consits of two domains (1 and 1) forming the peptide- binding region (PBR) and two other immunoglobulin like domains (2 and 2) forming the membrane proximal region. The class II proteins appear to form ...

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by saddy51
Sun Jan 04, 2015 10:39 am
Forum: Genetics
Topic: I have got some BIG issues! forgive the long post
Replies: 1
Views: 1778

Re: translation from DNA

That's a reasonable idea. I can imagine a ribosome directly binding to DNA to translate the DNA codons directly into protein. We can't really tell why that didn't happen, as much of the early development of life occurred far in the past and left no evidence ...

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by jonmoulton
Mon Sep 29, 2014 3:03 pm
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