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Bench

Bench

Origin: oe. Bench, benk, as. Benc; akin to Sw. Bank, dan baenk, Icel. Bekkr, os, D, & g. Bank. Cf. Bank, beach.

1. A long seat, differing from a stool in its greater length. Mossy benches supplied the place of chairs. (Sir W. Scott)

2. A long table at which mechanics and other work; as, a carpenter's bench.

3. The seat where judges sit in court. To pluck down justice from your awful bench. (Shak)

4. The persons who sit as judges; the court; as, the opinion of the full bench. See kings bench.

5. A collection or group of dogs exhibited to the public; so named because the animals are usually placed on benches or raised platforms.

6. A conformation like a bench; a long stretch of flat ground, or a kind of natural terrace, near a lake or river. Bench mark, a projecting course at the base of a building, or round a pillar, sufficient to form a seat.


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Re: Ligation and expression trouble

... that could be helpful. 1) Not that I left my ligase on the desk unattended but about your point 1-" if the ligase has been left on the bench for even a couple of minutes, it's pretty much lost." My question is: Ligase has 100% activity at room temperature then how can it go bad ...

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by solution
Wed Jul 08, 2009 9:16 pm
 
Forum: Molecular Biology
Topic: Ligation and expression trouble
Replies: 3
Views: 396

Re: Ligation and expression trouble

... you could try: 1. Throw away your T4 ligase and buffer and get new ones. this probably is not the problem, but if the ligase has been left on the bench for even a couple of minutes, it's pretty much lost. 2. Try higher insert:vector ratio. Also make sure to resuspend the insert in 30 microliters ...

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by MrMistery
Wed Jul 08, 2009 3:18 am
 
Forum: Molecular Biology
Topic: Ligation and expression trouble
Replies: 3
Views: 396

Anybody knows how stable is ssDNA??

... I labeled the probe with DIG, keep in 50%formamide and hybridization buffer with carrier DNA. I was so stupid that I left it overnight at my bench. Anybody has and information about how stable is ssDN at room temperature? especially in that buffer? Please help, my background in biochemistry ...

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by primary
Tue Jun 09, 2009 11:08 pm
 
Forum: Molecular Biology
Topic: Anybody knows how stable is ssDNA??
Replies: 2
Views: 173

Re: How to be "good at the bench"

Everyone struggles with lab skills to begin with. I improved a lot after doing a studentship at the end of my second year and have become even more stringent from working in industry. You never stop learning. It takes practise and being taught how to exercise procedures properly, for eg - holding a ...

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by Sepals
Tue May 26, 2009 10:18 am
 
Forum: General Discussion
Topic: How to be "good at the bench"
Replies: 3
Views: 1438

Re: How to be "good at the bench"

Thanks Canalon. I also read this book "At the bench" by Kathy Barker and wrote down things which were important for me. But nevertheless I thought it might be useful to somebody else also. I tried to attach the document, but I dont know ...

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by jangajarn
Sat May 23, 2009 7:15 pm
 
Forum: General Discussion
Topic: How to be "good at the bench"
Replies: 3
Views: 1438
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