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Dictionary » B » Beers law Beers lawBeers law The intensity of a colour or of a light ray is inversely proportional to the depth of liquid through which it is transmitted; it is concluded that the absorption is dependent upon the number of molecules in the path of the ray. Compare: beer-lambert law. ![]()
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Results from our forumRe: beers law concentration calculationno- Calculation of ε: Trp: 3 x 5600 = 16800 + Tyr: 6 x 1420= 8520 + Phe 1x197 = 25517 M-1 cm-1 0.57= 22517 c (1) c=.000022338 mol/ml c=( .000022338/2438)/ml = 9.1624 x 10 -6
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Re: beers law concentration calculationOk thanks- i had no idea concentration was expressed that way. So when talking about protein concentration is one always referring to weight concentration?
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Re: beers law concentration calculationOk I believe the reason I am confused is because in chemistry when we say "concentration" we usually mean molarity, or moles per unit volume. Here you are talking about concentration in terms of mass per volume, which I thought was density? Is it different for proteins than solutions?
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beers law concentration calculationHello, I need help understanding the units in this calculation. I am asked to provide the concentration of a protein and I am given the number of Trp and Tyr residues, 3 and 6, so I have calculated the molar absorptivity as 3*5600+6*1420= 25320 M^-1cm^-1. Is this the correct units for this value? Th...
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