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Beers law

Beers law

The intensity of a colour or of a light ray is inversely proportional to the depth of liquid through which it is transmitted; it is concluded that the absorption is dependent upon the number of molecules in the path of the ray.

Compare: beer-lambert law.


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Re: beers law concentration calculation

no- Calculation of ε: Trp: 3 x 5600 = 16800 + Tyr: 6 x 1420= 8520 + Phe 1x197 = 25517 M-1 cm-1 0.57= 22517 c (1) c=.000022338 mol/ml c=( .000022338/2438)/ml = 9.1624 x 10 -6

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by protchem12
Mon Jan 16, 2012 3:33 pm
 
Forum: Molecular Biology
Topic: beers law concentration calculation
Replies: 8
Views: 5990

Re: beers law concentration calculation

Ok thanks- i had no idea concentration was expressed that way. So when talking about protein concentration is one always referring to weight concentration?

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by protchem12
Fri Jan 13, 2012 1:52 pm
 
Forum: Molecular Biology
Topic: beers law concentration calculation
Replies: 8
Views: 5990

Re: beers law concentration calculation

Ok I believe the reason I am confused is because in chemistry when we say "concentration" we usually mean molarity, or moles per unit volume. Here you are talking about concentration in terms of mass per volume, which I thought was density? Is it different for proteins than solutions?

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by protchem12
Thu Jan 12, 2012 10:23 pm
 
Forum: Molecular Biology
Topic: beers law concentration calculation
Replies: 8
Views: 5990

beers law concentration calculation

Hello, I need help understanding the units in this calculation. I am asked to provide the concentration of a protein and I am given the number of Trp and Tyr residues, 3 and 6, so I have calculated the molar absorptivity as 3*5600+6*1420= 25320 M^-1cm^-1. Is this the correct units for this value? Th...

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by protchem12
Thu Jan 12, 2012 5:24 pm
 
Forum: Molecular Biology
Topic: beers law concentration calculation
Replies: 8
Views: 5990


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