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Beer-lambert law

Beer-lambert law

The equation A=ECL, where a is the absorbance at a given wavelength of light, E is the molar extinction coefficient, c is the concentration of the molar solution, and L is the length of the light path. In words, the equation means that the concentration of a substance in moles is proportional to the absorption of a given wavelength of light by a solution of the substance. The equation is used in the study of spectroscopy.


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Re: molar extinction coefficient

... the absorbance changes. The slope of their linear relationship (in the Beer's law range, A<~2) is the extinction coefficient. To lay it out explicitly, A=ec Y=mx Plot ...

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by 2468
Wed Nov 28, 2012 12:28 am
 
Forum: General Discussion
Topic: molar extinction coefficient
Replies: 10
Views: 6728

Re: molar extinction coefficient

... the absorbance changes. The slope of their linear relationship (in the Beer's law range, A<~2) is the extinction coefficient. To lay it out explicitly, A=ec Y=mx Plot ...

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by jonmoulton
Tue Nov 27, 2012 10:32 pm
 
Forum: General Discussion
Topic: molar extinction coefficient
Replies: 10
Views: 6728

Re: Measure of Cysteine concentration

... dilute and measure until you get an absorbance between 1 and 2 (in the Beer's law range). As Jack wrote, you need a molar extinction coefficient. Your path length is likely ...

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by jonmoulton
Thu Nov 15, 2012 4:23 pm
 
Forum: Molecular Biology
Topic: Measure of Cysteine concentration
Replies: 2
Views: 1402

Molar extinction coefficient troubles

Hi, i've recieved a piece of work to do with beer's law, i've tried to make some headway with it, but the results i get are always either far too large ...

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by Teleprion
Wed Aug 04, 2010 3:26 pm
 
Forum: Molecular Biology
Topic: Molar extinction coefficient troubles
Replies: 6
Views: 6302

Re: Why The Religious Minded Rule Science With Science

... Positive Surprised to see good things said by this poster about the 2nd Law of Thermodynamics Delia Posted: Jul 12 2008, 03:23 AM Negative Here's ... next negative! <Max> Posted: May 13 2008, 01:01 AM Negative -29 Beer w/Straw Posted: May 12 2008, 12:22 AM Negative look a neg Grumpy Posted: ...

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by GaryGaulin
Mon Aug 10, 2009 8:50 pm
 
Forum: Evolution
Topic: Why The Religious Minded Rule Science With Science
Replies: 20
Views: 52762
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