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Beer-lambert law

Beer-lambert law

The equation A=ECL, where a is the absorbance at a given wavelength of light, E is the molar extinction coefficient, c is the concentration of the molar solution, and L is the length of the light path. In words, the equation means that the concentration of a substance in moles is proportional to the absorption of a given wavelength of light by a solution of the substance. The equation is used in the study of spectroscopy.


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Re: Beer Lambert’s Law help..:(

Absorbance: let's say 0.2744 nm Extinction: 6220 l/mol/cm Path lenght (L): 1 cm 0.2744 / 62240 = 4.11 (the 1 get's cancelled out) now what? what's the next step? using international units of enzymes..

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by TwinkleK
Fri Feb 03, 2012 7:45 pm
 
Forum: Molecular Biology
Topic: Beer Lambert’s Law help..:(
Replies: 8
Views: 5282

Re: Beer Lambert’s Law help..:(

Yep, that's it:) Finding the concentation..the equation has to be arranged. Which I have:) I'm just not sure of the calculation steps.. (to find C)

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by TwinkleK
Fri Feb 03, 2012 7:01 pm
 
Forum: Molecular Biology
Topic: Beer Lambert’s Law help..:(
Replies: 8
Views: 5282

Re: Beer Lambert’s Law help..:(

The path lenght is 1 cm The molar extinction co efficient is 6220 l/mol/cm at 340 nm Stuck :( :(

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by TwinkleK
Fri Feb 03, 2012 6:46 pm
 
Forum: Molecular Biology
Topic: Beer Lambert’s Law help..:(
Replies: 8
Views: 5282

Re: Beer Lambert’s Law help..:(

Nice, Jack. For units, I'd add that you will need the path length for your spectrophotometer and the length unit from your extinction coefficient (hopefully these will match). The most likely light path length though your sample is 1 cm (if the spectrometer/colorimeter uses a 1 cm cell). Extinction ...

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by jonmoulton
Fri Feb 03, 2012 4:39 pm
 
Forum: Molecular Biology
Topic: Beer Lambert’s Law help..:(
Replies: 8
Views: 5282

Beer Lambert’s Law help..:(

... the graph (mol/min/L) Now I need to use the “Beer Lambert’s Law” to convert the gradient to velocity using the equation from ...

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by TwinkleK
Fri Feb 03, 2012 1:33 pm
 
Forum: Molecular Biology
Topic: Beer Lambert’s Law help..:(
Replies: 8
Views: 5282
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