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Beads

Beadsnake

(Science: zoology) a small poisonous snake of North America (Elaps fulvius), banded with yellow, red, and black.


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Basic Knowledge about DNA Extraction

... is often used for DNA extraction, because it can break apart cells in the early steps of extraction to make the DNA accessible. After adding glass beads to an eppendorph tube containing a sample of interest, the bead beater will vigorously vibrates thus the glass beads can physically break apart ...

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by 2444256703
Thu Jan 22, 2015 8:56 am
 
Forum: Genetics
Topic: Basic Knowledge about DNA Extraction
Replies: 0
Views: 752

In Vitro Transcription Translation

Dear Jonmoulton.The pull down certainly gives information on protein protein interaction [as done with the Beads to pull down.] I am not sure whether it made sense or sounds weird to you ::)

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by Shaanu
Sat Jun 08, 2013 3:53 pm
 
Forum: Molecular Biology
Topic: In Vitro Transcription Translation
Replies: 3
Views: 2672

How can a protein without a G-site bind to GSH?

Hello can someone help me understand my situation? i am new at this. I used GSH-Sepharose 4B beads on a cell lysate and got a single protein band on SDS-PAGE. I sent it for MALDI-TOF, the sequence came back with 3% coverage that has homolog with acetyl carrier protein. ...

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by hurzz
Mon Nov 12, 2012 12:34 pm
 
Forum: Molecular Biology
Topic: How can a protein without a G-site bind to GSH?
Replies: 11
Views: 4908

Why dialyse DMEM before protein purification (Ni-Affinity)?

... where then dialysed against a his-dialysis-buffer (50 mM NaH2PO4, 300 mM NaCl, pH 8). Afterwards Ni-NTA Agarose was added and finally these beads where put on a column. Some washing procedures followed (his-dialysis-buffer with either 10 mM imidazol or 50 mM imidazol) and in the end eluted ...

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by LittleBird
Sun May 20, 2012 3:43 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 3991

Re: Help: Coating Magnetic Beads with Vitamins

this article may help: http://www.sciencedirect.com/science/article/pii/S0079670007000512 cheers ... Chris

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by chrisd
Tue Mar 06, 2012 5:24 pm
 
Forum: Molecular Biology
Topic: Help: Coating Magnetic Beads with Vitamins
Replies: 1
Views: 2766
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