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Dictionary » B » Beads

Beads

Beadsnake

(Science: zoology) a small poisonous snake of North America (Elaps fulvius), banded with yellow, red, and black.


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In Vitro Transcription Translation

Dear Jonmoulton.The pull down certainly gives information on protein protein interaction [as done with the Beads to pull down.] I am not sure whether it made sense or sounds weird to you ::)

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by Shaanu
Sat Jun 08, 2013 3:53 pm
 
Forum: Molecular Biology
Topic: In Vitro Transcription Translation
Replies: 3
Views: 1403

How can a protein without a G-site bind to GSH?

Hello can someone help me understand my situation? i am new at this. I used GSH-Sepharose 4B beads on a cell lysate and got a single protein band on SDS-PAGE. I sent it for MALDI-TOF, the sequence came back with 3% coverage that has homolog with acetyl carrier protein. ...

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by hurzz
Mon Nov 12, 2012 12:34 pm
 
Forum: Molecular Biology
Topic: How can a protein without a G-site bind to GSH?
Replies: 11
Views: 2555

Why dialyse DMEM before protein purification (Ni-Affinity)?

... where then dialysed against a his-dialysis-buffer (50 mM NaH2PO4, 300 mM NaCl, pH 8). Afterwards Ni-NTA Agarose was added and finally these beads where put on a column. Some washing procedures followed (his-dialysis-buffer with either 10 mM imidazol or 50 mM imidazol) and in the end eluted ...

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by LittleBird
Sun May 20, 2012 3:43 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 2042

Re: Help: Coating Magnetic Beads with Vitamins

this article may help: http://www.sciencedirect.com/science/article/pii/S0079670007000512 cheers ... Chris

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by chrisd
Tue Mar 06, 2012 5:24 pm
 
Forum: Molecular Biology
Topic: Help: Coating Magnetic Beads with Vitamins
Replies: 1
Views: 2187

empty 1-2ml chromatography columns

Hi guys, I am looking for 1-2 ml empty chromatography columns into which I could load my Sephadex beads... Do you have any idea where can I get them? GE for example only sells 8 ml columns which are a bit too big for my needs... Arik

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by pushel
Sun Mar 04, 2012 2:53 pm
 
Forum: General Discussion
Topic: empty 1-2ml chromatography columns
Replies: 3
Views: 957
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