Beads

Hello can someone help me understand my situation? i am new at this. I used GSH-Sepharose 4B beads on a cell lysate and got a single protein band on SDS-PAGE. I sent it for MALDI-TOF, the sequence came back with 3% coverage that has homolog with acetyl carrier protein. ...

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... where then dialysed against a his-dialysis-buffer (50 mM NaH2PO4, 300 mM NaCl, pH 8). Afterwards Ni-NTA Agarose was added and finally these beads where put on a column. Some washing procedures followed (his-dialysis-buffer with either 10 mM imidazol or 50 mM imidazol) and in the end eluted ...

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... DNases work at 8 degree, they laughed at me. They usually make fun of me specially when I am not in the Lab. I want to add nuclear extract to my beads which have bounded oligo and shake it for 2hour at 4 degree. What is your idea? I am new PhD student.

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this article may help: http://www.sciencedirect.com/science/article/pii/S0079670007000512 cheers ... Chris

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Hi guys, I am looking for 1-2 ml empty chromatography columns into which I could load my Sephadex beads... Do you have any idea where can I get them? GE for example only sells 8 ml columns which are a bit too big for my needs... Arik

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