Bases

I agree with Jack. Polymerase adds bases by elongating from the 3' end of a nucleic acid strand which is bound in an antiparallel orientation with the template strand. I have not heard of priming the template backwards (parallel). I have never ...

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... The Homo Sapien is the longest DNA sequence. If the Homo Sapien gene has 1770 bases surely the overall aligned length should also be 1770, so why is it 1899? Also what exactly does the dots in the diagram represent?

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... of action of chaperon proteins are conserved between prokaryotes and eukaryotes True or False. 4)According to the Crick's wobble hypothesis what bases on the anticodon's 1st position can bind to the codon 3rd position?match the following: a)C ------------ 1)U,C or A b)A ------------- 2)G c)U ...

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Do not focus too much on Tm = 58. A primer of about 20 to 25 bases should do the trick. I always make sure there is a C or G at the end. Longer primers naturally give more trouble like hairpins. For checking my primers I like to use: http://basic.northwestern.edu/biotools/oligocalc.html

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... way would be to get new primer. All you have to do is shorten the primer with 71 degree Tm until it's around 60. You said it’s 23b, so you have 8 bases to play with.

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