Bands

If you don't mind losing the flexibility of scanning the spectrum or rapidly changing ex/em bands, you would build a 2-band fluorescence colorimeter. You'd need two emission filters and one or two excitation filters, arranged around your cuvette so that the excitation ...

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... in the ladder are from the same solution. In addition, I loaded 1X loading buffer dye in the empty wells. Any ideas? Also, I'm afraid that the bands will not be as prominent if I dilute it further. Thank you in advance!

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That is more clear for me thank you. Some bands are indeed a bit bigger than others, although it is not very clear!

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... periodicity? We are studying it by adding micrococcal nuclease within the nucleus and studying it by SDS-PAGE.The results we had, showed several bands around 6 (nucleosomes that were cut by the nuclease). I do not really know how to interpret the results caz I do not understand what they mean ...

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... areas." I don't think that the same primers that target the same location do end up in different areas. The difference in the masses of the bands produced is due to the difference in the number of repeats between individuals. Just looking at a single pair of primers, you target the same ...

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