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Banding pattern

Banding pattern

(Science: genetics) chromosomes stained with certain dyes, commonly quinacrine (Q banding) or giemsa (g banding), show a pattern of transverse bands of light and heavy staining that is characteristic for the individual chromosome.

The basis of the differential staining, which is the same in most tissues, is not understood: each band represents 5-10% of the length, about 10exp7 base pairs, although this is not true for polytene chromosomes in drosophila that show more than 4000 bands.


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Re: Basic electrophoresis digest banding pattern analysis....

... cleave; if concentration of your template is beyond the capacity of the enzyme that you have added then you will not get an expected fragmentation pattern.

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by whizliz
Wed May 04, 2011 10:03 am
 
Forum: Molecular Biology
Topic: Basic electrophoresis digest banding pattern analysis....
Replies: 5
Views: 3835

Basic electrophoresis digest banding pattern analysis....

I want to know more about banding pattern analysis...... lane1: standard makers (HindIII digest, sizes are 23.1,9.4,6.6,2.3,0.6,0.1kb) lane2: ...

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by scholesmu
Thu Apr 28, 2011 12:44 am
 
Forum: Molecular Biology
Topic: Basic electrophoresis digest banding pattern analysis....
Replies: 5
Views: 3835

Well...

... generated. I.E. I am fairly certain the insert is present based on the banding pattern generated from cutting the pBS-insert with EcoRI and HindIII. I actualy asked if XhoI ...

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by pez123
Thu May 24, 2007 9:49 pm
 
Forum: Molecular Biology
Topic: SalI is a jerk
Replies: 4
Views: 2750

politenic chromosomes?

... to determine the specific location of genes based on the morphology (banding pattern) of these chromosomes. But i don't really know much about this stuff "off the ...

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by LilKim
Mon Jan 29, 2007 6:08 am
 
Forum: Genetics
Topic: politenic chromosomes?
Replies: 4
Views: 2938

Subspecies comparison of the Genus: Corucia

... Gray describes Corucia zebrata zebrata (underlined) as having prominent banding. The bands are alternating dark brown-black/ green. The green portion ... spot pigmentation throughout the body in various sizes, numbers, and patterns. The spots may overlap. Dark green body coloration may occur with ...

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by Brian - LCRC
Sat Aug 26, 2006 1:15 pm
 
Forum: Evolution
Topic: Subspecies comparison of the Genus: Corucia
Replies: 1
Views: 9381
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