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Band cell

Band cell

(Science: pathology) immature neutrophils released from the bone marrow reserve in response to acute demand.


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Weird - shaped band on western blotting

... in this forum. My name is Laila and currently working with the hepatoma cells AH109A to see the cell cycle regulation protein. Recently I got a weird-shaped band on my western blot. It is like an upside-down of smiling face in each lane. I use the ...

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by namakulaila
Thu Oct 17, 2013 6:17 am
 
Forum: Molecular Biology
Topic: Weird - shaped band on western blotting
Replies: 2
Views: 1477

How can a protein without a G-site bind to GSH?

... my situation? i am new at this. I used GSH-Sepharose 4B beads on a cell lysate and got a single protein band on SDS-PAGE. I sent it for MALDI-TOF, the sequence came back with 3% coverage that has ...

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by hurzz
Mon Nov 12, 2012 12:34 pm
 
Forum: Molecular Biology
Topic: How can a protein without a G-site bind to GSH?
Replies: 11
Views: 3572

molecular cloning failure

... my master thesis in the cloning of keratinase sequence in competent cell.I tried several different methods and procedure for the cloning and ... concentration of the DNA but when run on agarosei could not see the band.. I have to stop my thesis at this point but I am unable to think What ...

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by siddharthsameer
Tue Oct 23, 2012 6:33 pm
 
Forum: Molecular Biology
Topic: molecular cloning failure
Replies: 3
Views: 1492

Re: restriction analysis

... the plasmid and then i have to to subclone it into E.coli TOF competent cell and then i have to plate it select for the colonies and then do the ... question is if i do by this process and I do the pcr Should I get the band on 1,4kb AND THEN I EXCISE IT AND CARRYOUT MY FURTHER PROCESS: IF i ...

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by siddharthsameer
Mon Jul 16, 2012 12:33 pm
 
Forum: Molecular Biology
Topic: restriction analysis
Replies: 14
Views: 7573

Re: Protein expression but no mRNA

... not responding sooner. The protein is indeed present in serum. A strong band is detected both in patients and in healthy controls. The molecular weight in serum is the same as seen in cell lysates. Maybe, the protein is simply bound to cell surface receptors?!? But ...

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by evamik
Thu Jun 07, 2012 8:02 am
 
Forum: Molecular Biology
Topic: Protein expression but no mRNA
Replies: 10
Views: 7105
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