Band 3 protein

... be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS? sorry, ...

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... be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS?

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Hi BIologists, I have a question, how can we know about the size of protein in the western blot? I know the full lenght, but after WB, I am seeing band with a different size! Thanks for your help.

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... and Native PAGE to verify my results. For SDS PAGE, there were 3 faint bands found and all of it are very near to each other. For Native PAGE, there ... loading well, I suspect it wasn't just my enzyme but it probably is a protein aggregate. How do I verify this i.e. is it only one protein or protein ...

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... i can do N-term sequencing. we don't have it here. i have abt 1.5mg of protein, ran on SDS-PAGE got a single band, but when i did 2DE i got 2 spots of the same MW but different pI. sent it for MALDI-ToF-ToF, ...

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