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Band 3 protein

Band 3 protein

a ubiquitous membrane Transport Protein found in the plasma membrane of diverse cell types and tissues, and in nuclear, mitochondrial, and golgi membranes. It is the major integral transmembrane protein of the erythrocyte membrane, comprising 25% of the total membrane protein and occurring at 1 million copies per cell. It exists as a dimer and provides a channel for the transport of anions across the membrane.


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Weird - shaped band on western blotting

... working with the hepatoma cells AH109A to see the cell cycle regulation protein. Recently I got a weird-shaped band on my western blot. It is like an upside-down of smiling face in each lane. I use the ...

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by namakulaila
Thu Oct 17, 2013 6:17 am
 
Forum: Molecular Biology
Topic: Weird - shaped band on western blotting
Replies: 2
Views: 1376

Re: In Vitro Transcription Translation

... sounds like the autoradiography is done directly on the gel. Since the protein contains the hot sulfur, you don't need a radiolabeled antibody to ... can infer the mass of the protein from its mobility (location of gel band). However, if you need to unambiguously prove the identity of the protein ...

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by jonmoulton
Wed Jun 05, 2013 10:59 pm
 
Forum: Molecular Biology
Topic: In Vitro Transcription Translation
Replies: 3
Views: 1975

Re:

... be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS? sorry, ...

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by Bio2013
Sat Feb 16, 2013 8:48 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 3274

Western Blot

... be mostly glycosylation or proteolysis, depending on the size of your band. Or the mobility of your protein is affected for some reason. Could you measure size of your protein on MALDI MS?

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by JackBean
Mon Feb 11, 2013 3:31 pm
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 3274

Western Blot

Hi BIologists, I have a question, how can we know about the size of protein in the western blot? I know the full lenght, but after WB, I am seeing band with a different size! Thanks for your help.

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by Bio2013
Wed Jan 30, 2013 7:00 am
 
Forum: Cell Biology
Topic: Western Blot
Replies: 6
Views: 3274
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