Baculovirus

Heya, I am considering making a baculovirus for protein expression. I haven't found anybody local who has any recent experience doing this. So I went online and did some browsing. Of course, all the companies' websites say how easy and fantastic ...

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... production. Just bear in mind that also eukaryotic cells can be used in similar manner; yeasts are often used, as are insect cells (with e.g. baculovirus vectors) and even human cells. But this is where the benefits of bacteria can be seen: they are much more simple and cheap to grow than ...

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Hi: Does anyone know how long time (hours - days) it will take if I want to clone a gene in Baculovirus system. and how long time it will take to express the proteins of that gene? The time is critical for me could anyone help?

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... TATA box, stop codon, for E coli; enhancers sometimes for mammalian cell expression; insect-specific upstream or downstream elements for baculovirus expression etc). You may induce expression especially when using E coli, but that's not amplification of the sequence. Induction is turning ...

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HI Can anyone tell me why is the frame of the inserted DNA important in bacterial expression system while it is not important for mammalian and baculovirus system?

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