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Baculovirus

Baculovirus

(Science: virology) viruses specialised as pathogens of lepidopteran larvae. Widely used as eukaryotic expression vectors for proteins requiring post translational modifications such as glycosylation, proteolytic cleavage and fatty acylation.


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Re:

... reagents that you have in the reaction. They are still at the same diluted factor but it gives your pcr a better chance at working. I know for my baculovirus work, that when I was using a volume of 20uL nothing would work but when I increased it to 50uL I was getting very good amplification. Also ...

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by antonyviridae
Wed Sep 07, 2011 2:13 pm
 
Forum: Molecular Biology
Topic: PLease Please I need help with my PCR! Greatly appreciated!
Replies: 8
Views: 9816

Baculovirus amplification in sf9 cells.

Hi, I have some doubts regarding virus amplification time. I have found instructions for working with sf9 cells and it is written there that incubation after cells infection with virus during amplification should be 72 hours (3days), because after that virus titer will decrease. But guys in our lab ...

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by polinazzz
Mon Jun 27, 2011 5:23 pm
 
Forum: Cell Biology
Topic: Baculovirus amplification in sf9 cells.
Replies: 1
Views: 2983

How do you overexpress and knock down a gene

To overexpress a gene of interest, maybe u can clone ur gene into Baculovirus. Baculovirus has a strong promoter which will actively transcribe the gene of interest. Maybe you can find the protocol of using Baculovirus. To knock down a gene, many different ...

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by magicsiew
Tue Nov 30, 2010 6:21 pm
 
Forum: Molecular Biology
Topic: How do you overexpress and knock down a gene
Replies: 2
Views: 13613

baculovirus expression systems

Heya, I am considering making a baculovirus for protein expression. I haven't found anybody local who has any recent experience doing this. So I went online and did some browsing. Of course, all the companies' websites say how easy and fantastic ...

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by grizzytove
Tue Jun 09, 2009 5:34 pm
 
Forum: Molecular Biology
Topic: baculovirus expression systems
Replies: 0
Views: 1009

Why is bacteria good?

... production. Just bear in mind that also eukaryotic cells can be used in similar manner; yeasts are often used, as are insect cells (with e.g. baculovirus vectors) and even human cells. But this is where the benefits of bacteria can be seen: they are much more simple and cheap to grow than ...

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by biohazard
Fri Feb 13, 2009 9:39 pm
 
Forum: Microbiology
Topic: Why is bacteria good?
Replies: 12
Views: 13942
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