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B lymphocyte

B lymphocyte

(Science: haematology, immunology) An immunologically important lymphocyte, produced by the bone marrow, that is responsible for the production of immunoglobulins, it is the precursor of the plasma cell.

The surface markers can be used to detect and differentiate chronic lymphocytic leukaemia from acute lymphocytic leukaemia. A lymphocyte derived from bone marrow that provides humoral immunity; it recognizes free antigen molecules in solution and matures into plasma cells that secrete immunoglobulin (antibodies) that inactivate the antigens.


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Reading Blood under a microscope

1) correct 2) just several red blood cells aggregated 3) probably some lymphocyte or something like that

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by JackBean
Mon Jan 30, 2012 11:39 am
 
Forum: Human Biology
Topic: Reading Blood under a microscope
Replies: 5
Views: 2190

CFSE proliferation assay

Dear Biohazard, First of all I got 2 separate populations (2 peaks) in lymphocyte population when stained thawed PBMCs without incubation of cells (I used 5 mM stock solution for 1 million cells and incubate for 10 min according protocol C34554, Molecular Probes ...

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by molbiol
Fri Nov 11, 2011 9:51 am
 
Forum: Cell Biology
Topic: CFSE proliferation assay
Replies: 3
Views: 1714

CFSE proliferation assay

... the staining. However, in the results from Flow Cytometry of day 0 samples, (i.e. freshly stained and not cultured) there are 2 separated picks in lymphocyte population. Does anyone encounter this problem before and what could be the reasons. And next question is that I tried to titrate the amount ...

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by molbiol
Tue Nov 08, 2011 10:29 am
 
Forum: Cell Biology
Topic: CFSE proliferation assay
Replies: 3
Views: 1714

Mitogenesis assay?

... concentrations of mitogen were added to the cell suspension and the plated suspensions were measured for absorbance to measure splenocyte/lymphocyte proliferation. When I drew curves for the two lectins, plotting concentration against absorbance, they were roughly ( very roughly ) a bell ...

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by sam2400
Mon Aug 09, 2010 12:41 pm
 
Forum: Microbiology
Topic: Mitogenesis assay?
Replies: 2
Views: 2745

Re: How's red blood cells look like under the microscope?

... very easy, all you need is some xylene, ethanol and H and E.. Once you have stained your sample, WBC are bigger and nucleated. The picture shows a lymphocyte and what looks like a monocyte in the lower half of the screen.

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by goonwolf420
Thu May 20, 2010 7:59 am
 
Forum: Cell Biology
Topic: How's red blood cells look like under the microscope?
Replies: 6
Views: 96234
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