Dictionary » A » Auxotrophic



Pertaining to an auxotroph.

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E.coli MC4100 thi- genotype

I'm trying to create a pspA reporter plasmid by linking the psp promoter using the thi- genotype of E.coli MC4100 as an auxotrophic marker, however I'm having 'a little' trouble finding out which gene has actually been deleted or mutated so that I can incorporate it into my plasmid. ...

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by julian
Tue Feb 24, 2009 3:50 am
Forum: Molecular Biology
Topic: E.coli MC4100 thi- genotype
Replies: 0
Views: 1053

help me with these question sum1 plz

... require an additional supplement in order to grow on minimal medium ( i.e. prototrophic) and was sensitive to antibiotic A (Ab A). E.coli Z was an auxotrophic mutant, requiring serine to be added to MINIMAL medium in order for growth to occur. It was also resistant to Ab A. Equal numbers of cells ...

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by sanj123
Tue Mar 28, 2006 2:45 pm
Forum: Molecular Biology
Topic: help me with these question sum1 plz
Replies: 2
Views: 1416

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