Attachment sites

... with another Arabidopsis gene that I am interested in. The cut sites are Hind 3 and Pst I, and the insertion is directional cloning. I cut ... results. Please help, and thanks in advance! If you can't download the attachment, here's a link to the image: http://img221.imageshack.us/my.php?image=colonypcr2mi9.jpg

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... that supports the hypothesis the the phenotype is due to knockdown of the targeted gene and not an off-target RNA. Only if the off-target RNA had sites where both of the oligos could bind would you see the dose-synergy effect on an off-target RNA; while there is some probability of both targets ...

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It's not simply one spot, each spot has a lot of attachment sites.

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... in the a)binding of ribosomes to mRNA b)shape of the A and P sites of ribosomes c) bonding of the anticodon to the codon d) Attachment of the amino acids to tRNAs e) both C and D

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... Messenger RNA is RNA that carries information from DNA to the ribosome sites of protein synthesis in the cell. Once mRNA has been transcribed from ... of protein synthesis during translation. It has sites for amino-acid attachment and an anticodon region for codon recognition that binds to a ...

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