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Attachment sites

Attachment sites

(Science: microbiology molecular biology) particular loci in both bacterial and phage dna molecules at which phage dna is integrated into the bacterial dna by recombination between these sites.


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PLease Please I need help with my PCR! Greatly appreciated!

... post it as well) and the following were my primers along with the rest of the PCR details that I followed. Primers (in brackets are restrictions sites) Forward Primer (NdeI) 5’ (c a t a t g) a t g g t g g a c t t g g c t a g g g t 3’ Reverse Primer (XhoI) 5’ (c t c g a g) t t a g c t g g a c ...

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by yasamino
Mon Aug 29, 2011 8:21 pm
 
Forum: Molecular Biology
Topic: PLease Please I need help with my PCR! Greatly appreciated!
Replies: 8
Views: 8884

Re: restriction enzyme question.

... you for your help so far :D...but one last thing (hopefully) regarding attachment of restriction sites to the end of the primers...so say this site is at the 5 end of primer...when the first ...

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by biology_06er
Sun Jun 19, 2011 1:28 pm
 
Forum: General Discussion
Topic: restriction enzyme question.
Replies: 9
Views: 4065

Help please! Directional cloning: colonies with WEIRD DNA?

... with another Arabidopsis gene that I am interested in. The cut sites are Hind 3 and Pst I, and the insertion is directional cloning. I cut ... results. Please help, and thanks in advance! If you can't download the attachment, here's a link to the image: http://img221.imageshack.us/my.php?image=colonypcr2mi9.jpg

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by walkthewok
Sun Nov 30, 2008 5:41 am
 
Forum: Molecular Biology
Topic: Help please! Directional cloning: colonies with WEIRD DNA?
Replies: 1
Views: 1946

Re: splicing MO vs translation MO

... that supports the hypothesis the the phenotype is due to knockdown of the targeted gene and not an off-target RNA. Only if the off-target RNA had sites where both of the oligos could bind would you see the dose-synergy effect on an off-target RNA; while there is some probability of both targets ...

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by jonmoulton
Wed Aug 20, 2008 4:57 pm
 
Forum: Molecular Biology
Topic: splicing MO vs translation MO
Replies: 14
Views: 10799

glucose transporters

It's not simply one spot, each spot has a lot of attachment sites.

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by mith
Fri May 09, 2008 1:38 pm
 
Forum: Molecular Biology
Topic: glucose transporters
Replies: 12
Views: 5073
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