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Assay

Assay

(Science: procedure) The determination of the amount of a particular constituent of a mixture or of the biological or pharmacological potency of a drug.


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Re: Lonza MycoAlert test

This assay is quite robust but like all products has a few pitfalls. Questions: 1.) are you using a multifunction plate reader with a luminescence setting or a dedicated cuvette luminometer? 2.) Are you using MycoAlert or MycoAlert ...

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by buzzbomb711
Fri Jul 25, 2014 6:51 am
 
Forum: General Discussion
Topic: Lonza MycoAlert test
Replies: 1
Views: 1558

mRNA vs. cell reporter assay

Hi, I have a GFP reporter cell line that drives GFP expression upon activation of a transcription factor. In theory, I can study transcription factor activation via the GFP signal, or by measuring the mRNA of the transcription factor. I wonder what are the pros and cons of each. Can anyone help me f...

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by aguy
Mon Jul 07, 2014 2:50 am
 
Forum: Cell Biology
Topic: mRNA vs. cell reporter assay
Replies: 2
Views: 693

DNA testing times

... this stuff these days, so you should find a better source for confirmation. In general the process involves: (1) sample preparation (2) running an assay (3) analysis The time required will depend on what sort of information you need and therefore what assay you would need to perform, which determines ...

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by jonmoulton
Fri Apr 11, 2014 2:47 pm
 
Forum: Genetics
Topic: DNA testing times
Replies: 2
Views: 1210

electrophoretic mobility shift assay (EMSA)

I am doing EMSA and after the first try having success, I could never reproduce my results: following the streptavidin-biotin-HRP detection, there are no bands on the blot paper. Compared to the first succesful try, the most striking difference I experience is that while at first, there was a bromop...

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by kk
Fri Nov 08, 2013 12:21 pm
 
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 1305

Transcrption factor-promoter (cis-element) interaction

How about EMSA then? electromobility shift assay also applicable for the same purpose more or less...

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by bravebeaker
Mon Aug 26, 2013 5:45 am
 
Forum: Molecular Biology
Topic: Transcrption factor-promoter (cis-element) interaction
Replies: 3
Views: 1321
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