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Assay

Assay

(Science: procedure) The determination of the amount of a particular constituent of a mixture or of the biological or pharmacological potency of a drug.


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electrophoretic mobility shift assay (EMSA)

I am doing EMSA and after the first try having success, I could never reproduce my results: following the streptavidin-biotin-HRP detection, there are no bands on the blot paper. Compared to the first succesful try, the most striking difference I experience is that while at first, there was a bromop...

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by kk
Fri Nov 08, 2013 12:21 pm
 
Forum: Molecular Biology
Topic: electrophoretic mobility shift assay (EMSA)
Replies: 1
Views: 645

Transcrption factor-promoter (cis-element) interaction

How about EMSA then? electromobility shift assay also applicable for the same purpose more or less...

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by bravebeaker
Mon Aug 26, 2013 5:45 am
 
Forum: Molecular Biology
Topic: Transcrption factor-promoter (cis-element) interaction
Replies: 3
Views: 829

Transcrption factor-promoter (cis-element) interaction

are you testing in plant or animal cells. I have been working with plant cells and the most common and easy way I guess is the reporter gene assay. the promoter in question is designed in a vector carrying the GUS reporter gene then you can measure GUS activity for instance...

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by bravebeaker
Thu Aug 22, 2013 3:31 am
 
Forum: Molecular Biology
Topic: Transcrption factor-promoter (cis-element) interaction
Replies: 3
Views: 829

Cell Signaling

... I have done this in my masters thesis but have been asked by many that what is the objective. I could only say that its one more confirmatory assay apart from IP and one can be ascertained of direct interaction of your proteins as this assay is cell free system assay. Can anyone give me a ...

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by Shaanu
Mon Jun 17, 2013 9:04 am
 
Forum: Cell Biology
Topic: Cell Signaling
Replies: 1
Views: 674

Getting Bacteria from an OD0.8 to an OD 0.1

... at an OD0.1? Thanks, biology_06er Exp: Incase you want to know why I need at OD0.1 it's because I want to add a certain about of bacteria to my assay..I know roughly how many are contained in an OD0.1 so from there I can add the correct volume ..

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by biology_06er
Sun Jun 16, 2013 12:15 pm
 
Forum: Microbiology
Topic: Getting Bacteria from an OD0.8 to an OD 0.1
Replies: 1
Views: 803
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