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Artefact

Artefact --> artifact

Something artificial, a distortion that does not reflect normal anatomy or pathology, not usually found in the body. For example: in radiology, the appearance on an x-ray of a surgical metal clip that obscures the clear view of an anatomical structure.


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Problem with transgenic mice

... spliced variants and clone and sequence them too. But beware that PCR often creates bizarre unnatural hybrid molecules which are entirely artefact. If non of this helps, either your technique is wrong, or cut your losses and redesign your primers. I think that covers A LOT OF it!

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by merv
Sun Oct 02, 2011 9:32 pm
 
Forum: Genetics
Topic: Problem with transgenic mice
Replies: 2
Views: 7008

PLease Please I need help with my PCR! Greatly appreciated!

... spliced variants and clone and sequence them too. But beware that PCR often creates bizarre unnatural hybrid molecules which are entirely artefact. If non of this helps, either your technique is wrong, or cut your losses and redesign your primers. I think that covers it!

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by merv
Sun Oct 02, 2011 5:00 pm
 
Forum: Molecular Biology
Topic: PLease Please I need help with my PCR! Greatly appreciated!
Replies: 8
Views: 9522

Re:

... The tongue is not itching, the brain just thinks so because the signal comes along a neuron innervating the tongue. And the itch is most likely an artefact signal that is induced by the physical pressure on the damaged neurons.

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by biohazard
Thu Oct 29, 2009 7:34 am
 
Forum: General Discussion
Topic: Question about nerves.
Replies: 4
Views: 1995

Re: Serious cloning issues - gel extractions?

... seen in the linear vector lane, with a smear below it. Does this indicate degradation? The band is at too high a molecular weight so might be an artefact of some kind. On the other hand the insert is also running about 500 bp too high according to my linear ladder. If the gel extracted vector ...

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by TaintedCherub
Wed Oct 21, 2009 3:46 pm
 
Forum: Molecular Biology
Topic: Serious cloning issues - gel extractions?
Replies: 23
Views: 20459

The Fiber Disease

No effect of a parasite on reproduction in stickleback males: a laboratory artefact? Candolin U, Voigt HR. Department of Biology, University of Turku, Finland. u.candolin@uea.ac.uk Experiments are often carried out in the laboratory under artificial conditions. ...

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by London
Tue May 09, 2006 3:16 pm
 
Forum: Human Biology
Topic: The Fiber Disease
Replies: 7403
Views: 5415733


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