Arabidopsis

... this is my first post on this website, so I am not quite proficient when using it (hence my very late reply!). I would want to overexpress it in arabidopsis seeds to observe germination rates under high stress conditions etc. I have suggested cloning it (with introns in) to my supervisor but ...

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... proteins in rice genome and they were numbered as they were discovered. (or they could be number in accordance to paralogs in other plant, e.g. Arabidopsis)

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I have been trying very very hard to clone the ligase 6 gene in Arabidopsis overexpress it. However, it has been proving very difficult due to the high amount of splicing intermediates. I have so far used varying primers on the cDNA of arabidopsis in order ...

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APETALA is not only known in Arabidopsis.. it is a flowering gene..so it should be presented in angiosperm.. and i hv few sequence of apetala from other species..unfortunately its not enough to design a good primer

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So? I'm sure you could find such examples also in animals. Probably. My point is APETALA is only known in Arabidopsis and thus it's probably totally different from Hibiscus.

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