Aptamer

1. Does the entire nitrocellulose pad need to be pre-treated? (i.e. treated before aptamers are spotted onto the paper for fixation) And if so, with what? Don't know, sorry. 2. I was recommended to pre-treat my conjugate pad with either 10% sucrose, or a sucrose/borate ...

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... signal for the test. To explain a little more, I have a small nitrocellulose membrane, and I want to detect VEGF in sample, by using a anti-VEGF aptamer to capture it. My setup has an input where I place in VEGF sample and it wicks up and through a conjugate pad (made of fiber glass) that is ...

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