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Aptamer

Aptamer

(Science: molecular biology) a double stranded dna or single stranded rna molecule that bind to specific molecular targets, such as a protein or metabolite.

Aptamers (from the Latin aptus - fit, and Greek meros - part) are oligonucleotide or peptide molecules that bind to a specific target molecule. Aptamers are usually created by selecting them from a large random sequence pool, but natural aptamers also exist in riboswitches. Aptamers can be used for both basic research and clinical purposes as macromolecular drugs. Aptamers can be combined with ribozymes to self-cleave in the presence of their target molecule. These compound molecules have additional research, industrial and clinical applications.

More specifically, aptamers can be classified as:

DNA or RNA or RNA aptamers. They consist of (usually short) strands of oligonucleotides. Peptide aptamers. They consist of a short variable peptide domain, attached at both ends to a protein scaffold.


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Re: Lateral Flow Assay

1. Does the entire nitrocellulose pad need to be pre-treated? (i.e. treated before aptamers are spotted onto the paper for fixation) And if so, with what? Don't know, sorry. 2. I was recommended to pre-treat my conjugate pad with either 10% sucrose, or a sucrose/borate ...

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by jonmoulton
Thu Mar 08, 2012 6:55 pm
 
Forum: Cell Biology
Topic: Lateral Flow Assay
Replies: 1
Views: 2593

Lateral Flow Assay

... signal for the test. To explain a little more, I have a small nitrocellulose membrane, and I want to detect VEGF in sample, by using a anti-VEGF aptamer to capture it. My setup has an input where I place in VEGF sample and it wicks up and through a conjugate pad (made of fiber glass) that is ...

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by knclayton
Wed Mar 07, 2012 11:03 pm
 
Forum: Cell Biology
Topic: Lateral Flow Assay
Replies: 1
Views: 2593


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