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Antibody

Definition

noun, plural: antibodies

Any of the numerous Y-shaped gamma globulin proteins found in the blood or lymph, and produced by B cells as an immune defense against foreign agents (antigens). Each antibody has a region that binds specifically to a particular antigen which it neutralizes. It is typically made up of large heavy chains and small light chains.


Supplement

Antibodies are grouped based on their mode of action. Some of which are as follows:

Antibodies may be produced by direct exposure to foreign agents (active immunity). They may also be passed on from mother to offspring via milk (passive immunity).


Word origin: German Antikörper: from Latin anti- + körper (body).

Abbreviation: Ab

Synonym:

Compare: antigen

Related terms:


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Results from our forum


Stem Cells

... surface markers (cluster of differentiation; CD) that can also be used to track down these cells, either with flow cytometry or other monoclonal antibody/fluorescence-based assays. The problem is that the stem cells are usually very rare and thus difficult to detect, and in many cases the CD ...

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by biohazard
Tue May 14, 2013 6:56 am
 
Forum: Molecular Biology
Topic: Stem Cells
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Views: 335

ELISA

ELISA protocols changes according to the spesific antigen or the antibody investigated. It also changes according the antigen or antibody whether you use monoclonal or policlonal. What are you investigating specifically? And for what purpose do you need?

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by gislab03
Sat May 04, 2013 2:11 pm
 
Forum: Molecular Biology
Topic: ELISA
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Two-color in situ help

Problem seems to be solved. Not sure which step went wrong but the second signal began to appear when I diluted the second antibody 2X more and used less incubation time for the TSA reaction (about 3 min).

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by vastgenome
Sat Mar 09, 2013 4:21 pm
 
Forum: Molecular Biology
Topic: Two-color in situ help
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Views: 886

Re: Difference between ICC and IF

... so that is probably enough of a name. However, you could specify the antibodies, for example: immmunihistochemistry using goat anti-VEGFR primary antibody and anti-goat-IgG secondary antibody conjugated to horseradish peroxidase.

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by jonmoulton
Mon Feb 25, 2013 5:11 pm
 
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 7
Views: 9262

Re: Difference between ICC and IF

Typically you would use an antibody to bind to the protein of interest and then a secondary antibody carrying a fluorescent tag to detect the first antibody. The two-antibody approach is cheaper than making a custom-labelled fluorescent ...

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by jonmoulton
Wed Feb 20, 2013 5:20 pm
 
Forum: Molecular Biology
Topic: Difference between ICC and IF
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