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Dictionary » A » Antibiotic

Antibiotic

Antibiotic

(Science: pharmacology) a chemical substance produced by a microorganism which has the capacity, in dilute solutions, to inhibit the growth of or to kill other microorganisms.

antibiotics that are sufficiently nontoxic to the host are used as chemotherapeutic agents in the treatment of infectious diseases of man, animals and plants.

Origin: gr. Bios = life Of or relating to antibiotic drugs.A chemical substance derivable from a mold or bacterium that kills microorganisms and cures infections; when antibiotics were first discovered they were called wonder drugs.Antibiotics are used in medicine due to their capability of inhibiting or neutralising alien agents that enter the human body. Antibiotics are naturally produced by organisms as a means of defense and hence survival. Through natural selection, organisms that fall prey to antibiotics begin to develop a resistance, and the effects of the antibiotics diminish over time until the organisms are immune to its effect.


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Electroporation

... I don't think its that they are being incubated too long. What might be the cause of this? The only thing I can think might be the problem is her antibiotic being degraded/the wrong concentration? (ampicillin) Any other ideas would be gratefully received

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by lilruthc
Tue Feb 19, 2013 12:19 pm
 
Forum: Molecular Biology
Topic: Electroporation
Replies: 0
Views: 132

ligation error

... done ligation of xylanase gene into pRSET B and pACYCDUET-1 vector next , i incubate onto luria bertani (i use E. coli DH5a for host cell ) with antibiotic (amp50 for pRSET B, cam30 for pACYCDUET-1) at 37'C overnight. on the next day, i found that only pRSET B have white colonies, but pACYCDUET-1 ...

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by nekonara
Sun Feb 17, 2013 2:48 pm
 
Forum: Molecular Biology
Topic: ligation error
Replies: 1
Views: 171

Re: Markerless mutants using suicide vector

... we had actually successfully applied this method to our bacteria.. once (it couldn't be reproduced!). However, our linear KO DNA also contain an antibiotic cassette that we suspect may introduce some polar effect on downstream genes, so we are trying to avoid that route. If we try to, for example, ...

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by dhkwak1
Wed Dec 19, 2012 1:04 am
 
Forum: Genetics
Topic: Markerless mutants using suicide vector
Replies: 2
Views: 531

Cloning/Transformation question

... suggested (transform using water in place of vector) would be valid negative control, but essentially useless and uninformative - unless your antibiotic does not work anymore. The other suggestion - transformation using wrong plasmid (another antibiotic resistance) - is also wrong and has ...

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by Cat
Sat Dec 01, 2012 12:50 am
 
Forum: Molecular Biology
Topic: Cloning/Transformation question
Replies: 4
Views: 783

Cloning/Transformation question

you can either transform them with nothing or transform with plasmid with resistance to other antibiotic and then plate on two plates

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by JackBean
Fri Nov 30, 2012 10:12 am
 
Forum: Molecular Biology
Topic: Cloning/Transformation question
Replies: 4
Views: 783
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