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Anthrax

Anthrax

(Science: disease, microbiology) An infectious bacterial zoonotic disease usually acquired by ingestion of bacillus anthracis or its spores from infected pastures by herbivores or indirectly from infected carcasses by carnivores.

It is transmitted to humans usually by contact with infected animals or their discharges (agricultural anthrax) or with contaminated animal products (industrial anthrax). Anthrax is classified by primary routes of inoculation as: cutaneous, gastrointestinal and inhalational.

Synonym: charbon, milzbrand and splenic fever.


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enzyme inhibition assay

Hello everyone, I need some help to interpret my results...I'm working with anthrax lethal factor, which is a Zn2+ dependent metalloproteinase. I would like to calculate Ki of an inhibitor using a FRET inhibition assay.The FRET assay are run for 30 minutes at ...

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by claire84
Thu Jan 12, 2012 11:15 am
 
Forum: Molecular Biology
Topic: enzyme inhibition assay
Replies: 0
Views: 534

Re: HELP! Identification of unknown gram-positive bacterium!?

... thought could be the answer to my shape dilema. From what I've read all criteria match for Bacillus anthracis but I'm positive she didn't give me anthrax. =] Currently I'm leaning the most toward Corynebacterium diptheriae or Corynebacterium xerosis. Would this be a step in the right direction? ...

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by MGremillion
Sat Apr 09, 2011 9:50 pm
 
Forum: Microbiology
Topic: HELP! Identification of unknown gram-positive bacterium!?
Replies: 3
Views: 3784

enzymatic inhibition assay

Hello, I am new to your forum. I have to test the inhibition potency of same potential inhibitors against a metalloprotease, known as anthrax lethal factor, by means of a spectrophotometric assay. The enzymatic reactions were performed in 250 mM Na2HPO4, 150 mM NaCl, pH=7.4 at 25°C with a ...

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by claire84
Mon Aug 23, 2010 2:00 pm
 
Forum: Molecular Biology
Topic: enzymatic inhibition assay
Replies: 2
Views: 2177

Re: sterilization in microwave oven

... placing the syringes in heat-trapping ceramic bowls. Using a dose of Bacillus cereus dried on an envelope as a substitute for mail contaminated by anthrax spores, Bitton said he found he could kill 98 percent of the spores in 10 minutes by microwaving the paper – suggesting, he said, one possible ...

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by sciencenaturally
Tue Jan 19, 2010 2:17 am
 
Forum: Microbiology
Topic: sterilization in microwave oven
Replies: 28
Views: 81336

Re: Important unknown nanotech within humans

... included lectures on converting ordinary items into lethal biological weapons. He provided samples of virulent, designer strains of cholera, anthrax, botulism, plague, and malaria, as well as a bacteria he claimed had been mutated to be “pigment specific” for the white minority government ...

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by vincio
Mon Feb 16, 2009 2:36 am
 
Forum: Molecular Biology
Topic: Important unknown nanotech within humans
Replies: 32
Views: 124911
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