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Anther culture

a plant culturing technique in which immature pollen is made to divide andgrow into tissue (either callus or embryonic tissue) in either aliquidmedium or on solid media. Pollen-containing anthers are removed from aflower and put in a culture medium, some microspheres survive and developinto tissue. If embryonic tissue develops, its put in a medium favorablefor shoot and root development, if its callus tissue, its put in asolution of hormones that will spur it to differentiate and develop shootand root tissue.