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Anther culture

Anther culture

a plant culturing technique in which immature pollen is made to divide andgrow into tissue (either callus or embryonic tissue) in either aliquidmedium or on solid media. Pollen-containing anthers are removed from aflower and put in a culture medium, some microspheres survive and developinto tissue. If embryonic tissue develops, its put in a medium favorablefor shoot and root development, if its callus tissue, its put in asolution of hormones that will spur it to differentiate and develop shootand root tissue.

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Re: sporophytic haploids....is it true?

... (q.v.). The frequency of haploid plants can be greatly increased using anther culture (q.v.). Homozygous diploid plants can then be generated by treatment with colchicine ...

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by sara135
Tue Jul 12, 2011 8:23 am
Forum: Botany Discussion
Topic: sporophytic haploids....is it true?
Replies: 6
Views: 10082

DNA restriction map drawing...

1. A 1 in 4 dilution of an overnight culture of E coli has an absorbance (590nm) of 0.25 How much overnight culture must ... or linear fragment? how could i locate the site unambigously with respect to one anther? any uncertainty with respect to the map?

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by scholesmu
Mon Apr 25, 2011 10:23 pm
Forum: Molecular Biology
Topic: DNA restriction map drawing...
Replies: 1
Views: 1997

Genetics related MCQ

... process cld produce a large number of genetically identical plants? A anther culture B embryo culture C pollen cultue D protoplast culture My attempts: For 1,i chose D ...

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by Navin
Fri Nov 30, 2007 9:20 am
Forum: Genetics
Topic: Genetics related MCQ
Replies: 2
Views: 10760

Hapliod plants

Hi all,.. What if we manage to get a haploid plant via anther culture of F1 tomato hybrid..t hen the chromosome number is doubled by using colchicin.. does this ...

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by salsola
Mon Nov 21, 2005 9:30 pm
Forum: Genetics
Topic: Hapliod plants
Replies: 1
Views: 2419

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