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Annexin vi

Annexin vi

protein of the annexin family with a probable role in exocytotic and endocytotic membrane events.


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Cells hyper-sensitive to treatment after 48 hour culture

... thawed out cells (P11) frozen before these issues arose and yet these suffer in the same manner. Control untreated cells show no signs of dying (annexin V + propidium iodide staining) and are morphologically healthy. I've bought and made up new batches of media, ensured my supplements were the ...

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by danint
Thu Sep 20, 2012 2:20 pm
 
Forum: General Discussion
Topic: Cells hyper-sensitive to treatment after 48 hour culture
Replies: 0
Views: 733

Microparticles and flow cytometry

... So far I've worked out that it shows microparticles (I think!) and I know that more side scatter means a more complex cell and that Annexin binds to phosphatidylserine but I'm not sure how to interpret it any further than that. I've been told it comes from someone with a disease ...

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by Jemi
Tue Feb 21, 2012 3:30 pm
 
Forum: Molecular Biology
Topic: Microparticles and flow cytometry
Replies: 5
Views: 1819

FACS issue for Apoptosis?! Propidium Iodide Vs Annexin V-Cy5

... and Late Apoptotic and Dead cells post treatment with a particular cytotoxic agent and analyze using FACS (BD FACS CANTO 2). Propidium Iodide Vs Annexin V - Cy5 1. I am using 2 step Annexin V detection (We dont have Annexin V-FITC). Will this work effectively compared to Annexin-FITC. (rabbit ...

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by pjberlin
Thu May 13, 2010 2:34 pm
 
Forum: Molecular Biology
Topic: FACS issue for Apoptosis?! Propidium Iodide Vs Annexin V-Cy5
Replies: 0
Views: 2576

Research assignment

... by the sciencetist working within the firm. iii. Identify what kind of cell based assays/analysing methods which is used (e. g. apoptose. FACS, annexin, GFP) iv. Identify what cells are being used (e. g. jurkat, hela cells) What approach should I have to this assignment.. I've tried to go through ...

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by Lerhat
Sun May 03, 2009 6:50 pm
 
Forum: General Discussion
Topic: Research assignment
Replies: 0
Views: 1277

Lymphocyte Overnight Culture parameters

... I think you should do is to use the background signal from your control cultures (you need a control culture from every PBMC donor) as seen from annexin V and PI stains, and then compare this to your test cultures. You can then calculate a relative index of oxidative stress (or whatever you want ...

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by biohazard
Thu Nov 27, 2008 9:33 am
 
Forum: Molecular Biology
Topic: Lymphocyte Overnight Culture parameters
Replies: 5
Views: 8577
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