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Anneal

Anneal

The sustained heating of a material such as steel or glass at aspecific high temperature, followed by gradual cooling, this is done to eliminate weakness or to produce other qualities.

The pairing of complementary dna or rna sequences, via hydrogenbonding, to form a double-stranded molecule. Mostoften used to describe the binding of a short primer or probe.


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Annealing Temparature

The primers obviously contain added restriction sites and the matching part is quite short. I would anneal the first cycle at 50°C and the rest at 65°C. Denaturation for 10s will be enough, but a bit higher temperature 95-98°C might be better. Elongation depends on polymerase, ...

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by vladCH
Sat Feb 22, 2014 7:54 pm
 
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 4
Views: 350

PCR: Low Primer Annealing Temperature Explanation?

... I understand that the primers have a specific temperature in which they best function and that a temperature which is too low will cause them to anneal to non-specific locations of the DNA molecule. However, I was a little confused behind the reason as to why this happened. I've heard some explanations/theories ...

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by AciesEdge
Sat Oct 06, 2012 10:49 am
 
Forum: Genetics
Topic: PCR: Low Primer Annealing Temperature Explanation?
Replies: 4
Views: 6403

Re: Help - Why can't DNA be parallel?

Nice question :) Unfortunately, 3D picture would not help here. I was thinking, why couldn't DNA anneal in parallel configuration after denaturation in vitro . The answer is pretty simple - because it is not capable to do so. Look on this picture http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/B/BasePairing.gif ...

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by keetner
Tue Oct 02, 2012 9:07 pm
 
Forum: Molecular Biology
Topic: Help - Why can't DNA be parallel?
Replies: 7
Views: 3927

Re: Help - Why can't DNA be parallel?

Nice question :) Unfortunately, 3D picture would not help here. I was thinking, why couldn't DNA anneal in parallel configuration after denaturation in vitro . The answer is pretty simple - because it is not capable to do so. Look on this picture http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/B/BasePairing.gif ...

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by JackBean
Sun Sep 30, 2012 10:01 pm
 
Forum: Molecular Biology
Topic: Help - Why can't DNA be parallel?
Replies: 7
Views: 3927

Sequencing Primers

... One method is to synthesize a single stranded piece of DNA with a radioactive or fluorescent label as a probe. In ligation, the probe would only anneal to complimentary regions and identifies the presence of the known sequence. Maxam-Gilbert sequencing provides another possibility. In this method, ...

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by DougB
Mon Apr 23, 2012 6:25 pm
 
Forum: Genetics
Topic: Sequencing Primers
Replies: 4
Views: 2636
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