Anneal

Hi! Could you describe the experiment more detailed? where do the primers anneal? why are there two bands? did you use more than 2 primers? which polymerase did you use (proofreading or not)? does a primer anneal to the mutated site? I don't think that contamination ...

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No. It'll anneal any broken strand. The vector is prevented from self-ligation by alkaline phosphatase.

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... of RNA which contains a self-complementary region, and after the self-complementary region has been transcribed the two complementary sequences anneal together to form the hairpin. In the nucleus, the hairpin is cleaved by Drosha releasing a stem-loop. The hairpin is exported into the cytoplasm ...

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It helps to consider both strands. Bam H1 gGATCC CCTAGg Bgl II aGATCT TCTAGa Bam H1 end g CCTAG Bgl II end a TCTAG Flip the Bgl II end over and anneal it to the Bam H1 site. Combined site after annealing gGATCT CCTAGa Does this site match either the Bam H1 or the Bgl II recognition sequence? What ...

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... ggaagtgcca 481 tgagcaggcc tcttatacat tttggcaatg actatgagga ccgttactat cgtgaaaaca 3' -A diagram indicating where any primers or probes would anneal to the above sequence (you can annotate the sequence above). -A list of any reagents needed, i.e. oligonucleotides, enzymes, etc. -A statement ...

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