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Analyte

Analyte

(Science: chemistry) The substance being measured in an analytical procedure.


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Re: what is gain in fluorescence detector

When you increased the analyte concentration, did the machine calibrate gain before the first cycle?

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by jonmoulton
Fri Oct 12, 2012 2:40 pm
 
Forum: Molecular Biology
Topic: what is gain in fluorescence detector
Replies: 6
Views: 2719

Re: Is protein degrade by UV ?

... using UV to sterilize, you are trying to damage/kill microbes by messing with their proteins and nucleic acids. Yes, that can also mess with your analyte protein.

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by jonmoulton
Fri Aug 31, 2012 4:04 pm
 
Forum: Cell Biology
Topic: Is protein degrade by UV ?
Replies: 3
Views: 3361

surface plasmon resonance

... interaction between two proteins. I am using CM5 sensor chips for the immobilization of one of my protein (ligand protein)and flowing another analyte protein (Molecular weight 20KD) through the flow channel. I am getting good immobilization of my ligand protein as detected by resonance units ...

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by akaman81
Sun Mar 27, 2011 7:13 pm
 
Forum: Molecular Biology
Topic: surface plasmon resonance
Replies: 1
Views: 1314

Re: Nanodrop

... through the sample. More traditional spectrophotometers use cuvettes to contain the sample and generally require considerably more volume of the analyte solution. The advantage of the nanodrop system is that by using only a drop of analyte solution, it conserves sample.

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by jonmoulton
Mon Feb 14, 2011 4:17 pm
 
Forum: Molecular Biology
Topic: Nanodrop
Replies: 3
Views: 8061

Running SDS PAGE for the first time!

... of my protein after running it on the gel, however I need to be sure that my protein separates cleanly (although I highly suspect it will not). My analyte is actually a single protein, however it is being cross-linked to a polymer, and cross-linking may occur multiple times per protein. Anyway, ...

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by dhkwak
Wed Feb 03, 2010 4:23 pm
 
Forum: Molecular Biology
Topic: Running SDS PAGE for the first time!
Replies: 9
Views: 4660
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