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Dictionary » A » Amplify

Amplify

Definition

verb

(1) To increase in number, effect, size, volume, amplitude of, etc.

(2) To make larger, greater, stronger; to enlarge.

(3) To exaggerate.


Supplement

Word origin: late ME amplifyen < MF amplifier < L amplificāre (to increase, augment).


Related forms: amplifies (verb: simple present), amplified (verb: simple past), amplifying (verb: present participle), amplification (noun), amplifier (noun).

Related term: gene amplification


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Primer problem

Hi I designed a specific primers and blast it to amplify my gene. this primer amplifies a gDNA of the gene. I used dreamtaq kit and its protocol but unfortunately the expected product (2054 bp) was not there and what I got is several bands below ...

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by roniadam
Sat Sep 05, 2009 3:10 am
 
Forum: Molecular Biology
Topic: Primer problem
Replies: 2
Views: 32

Re: I get short smears trying to amplify tomato DNA, pleaseHelp

I have ordered samples of plant DNA extraction kits from both MoBio and Epicenter. I am looking forward to try those. I noticed that on the gel I ran for my genomic extract that there was much more intensity of bands that were very small (~50-400)bp compared to larger in tact fragments present. I th...

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by tomato
Tue Jul 28, 2009 7:41 pm
 
Forum: Molecular Biology
Topic: I get short smears trying to amplify tomato DNA, pleaseHelp
Replies: 5
Views: 173

Re: I get short smears trying to amplify tomato DNA, pleaseHelp

Yes, I have tried both the same temperature in addition to trying slightly lower and slightly higher. No difference. But as my standard reaction goes I use the same as the temperature published.

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by tomato
Fri Jul 24, 2009 12:59 pm
 
Forum: Molecular Biology
Topic: I get short smears trying to amplify tomato DNA, pleaseHelp
Replies: 5
Views: 173

I get short smears trying to amplify tomato DNA, pleaseHelp

... published in many papers with no problems. I am following their protocols exactly. Any suggestions to what might be going on with my attempt to amplify my gene of interest? Thanks

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by tomato
Thu Jul 23, 2009 9:27 pm
 
Forum: Molecular Biology
Topic: I get short smears trying to amplify tomato DNA, pleaseHelp
Replies: 5
Views: 173

primer design - please help

you could blast it and find out what the dna sequence for it is, assuming you want to amplify it directly from a person. In any way, you do need the dna sequence

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by MrMistery
Tue May 05, 2009 11:59 pm
 
Forum: Genetics
Topic: primer design - please help
Replies: 3
Views: 581
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