Dictionary » A » Amplification

Amplification

Definition

noun

(1) The act or result of increasing in size or effect.

(2) An increase in the frequency of a gene or chromosomal region, as a result of replicating a DNA segment by in vivo or in vitro process, such as by gene duplication or polymerase chain reaction, respectively.


Supplement

Word origin: Latin amplificatio < amplificare (“‘to amplify’”).
Related form: amplify (verb)

Related term: gene amplification


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


amplification in no RT control

... been isolated by trizol method and subsequently 'cleaned' on column and finally been DNase treated prior to reverse transcription. I am getting amplification in all of my no RT controls (4 treatments and an untreated), although this is occurring ~20 cycles later than RT+ samples. I am getting ...

See entire post
by biomart
Fri Mar 20, 2015 1:58 am
 
Forum: General Discussion
Topic: amplification in no RT control
Replies: 0
Views: 38

translation from DNA

What jonmoulton wrote is true (regulation on RNA level), but further there is amplification of the signal (there can be thousands copies of each mRNA, each giving rise to hundreds of protein molecules), also, during replication of DNA the protein synthesis would ...

See entire post
by JackBean
Tue Sep 30, 2014 8:16 am
 
Forum: Molecular Biology
Topic: translation from DNA
Replies: 4
Views: 5285

Re: Any ideas for vaccine development or anti-viral drugs???

... S, Warfield KL, Swenson DL, Stein DA, Smith JL, Gamble CS, Kroeker AD, Iversen PL, Bavari S, Muhlberger E. VP35 Knockdown Inhibits Ebola Virus Amplification and Protects against Lethal Infection in Mice. Antimicrob Agents Chemother. 2006 Mar;50(3):984-93. Phosphorodiamidate morpholino oligomers ...

See entire post
by jonmoulton
Mon Sep 15, 2014 3:04 pm
 
Forum: Cell Biology
Topic: Any ideas for vaccine development or anti-viral drugs???
Replies: 2
Views: 12545

Re: An interesting PCR smearing...I dont know what to do :(

... 3 different primers to amplify 3 different exons of a gene . 1 of the exon is amplifying very well ( a single bright band ) but the other two exon amplification is not coming properly.and this has occured recently. ( even though the PCR reaction was standardized earlier ) I have used same reagents ...

See entire post
by priyah
Sat Jun 28, 2014 5:20 pm
 
Forum: Molecular Biology
Topic: An interesting PCR smearing...I dont know what to do :(
Replies: 9
Views: 8772

A question regarding PCR

... will be much more than 7 bp and it will be on both sides. Yes, the polymerase will amplify the chain until the conditions change (i.e. end of amplification step) or until it falls off (which may happen with very long amplicons, that's why you need special polymerase for PCR of long pieces). ...

See entire post
by JackBean
Sat Jan 18, 2014 2:36 pm
 
Forum: Molecular Biology
Topic: A question regarding PCR
Replies: 1
Views: 2206
View all matching forum results

This page was last modified 09:13, 10 December 2008. This page has been accessed 15,763 times. 
What links here | Related changes | Permanent link