Dictionary » A » Amplification

Amplification

Definition

noun

(1) The act or result of increasing in size or effect.

(2) An increase in the frequency of a gene or chromosomal region, as a result of replicating a DNA segment by in vivo or in vitro process, such as by gene duplication or polymerase chain reaction, respectively.


Supplement

Word origin: Latin amplificatio < amplificare (“‘to amplify’”).
Related form: amplify (verb)

Related term: gene amplification


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A question regarding PCR

... will be much more than 7 bp and it will be on both sides. Yes, the polymerase will amplify the chain until the conditions change (i.e. end of amplification step) or until it falls off (which may happen with very long amplicons, that's why you need special polymerase for PCR of long pieces). ...

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by JackBean
Sat Jan 18, 2014 2:36 pm
 
Forum: Molecular Biology
Topic: A question regarding PCR
Replies: 1
Views: 455

TLDA screening analysis

... TLDA. We have a Workflow in R for analyzing exported .txt files, but we haven't software to analyze all the plates at once in order to analyze the amplification curves. There is software to scan files .sds (Applied Biosystems qPCR device), but not files .eds (new file format exported by Viia7). ...

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by laurentPre
Fri Jan 17, 2014 12:59 pm
 
Forum: Molecular Biology
Topic: TLDA screening analysis
Replies: 4
Views: 470

Re: Sound and how the brain reacts to it

... how the weakest audible sounds are detected, since they excite vibrations below the thermal noise background in the ear. The answer may be active amplification. Previous experiments performed in vitro found that bundles oscillate spontaneously from an internal driver, and this motion could synchronize ...

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by GremlinGnome422
Mon Jul 01, 2013 7:25 pm
 
Forum: Human Biology
Topic: Sound and how the brain reacts to it
Replies: 88
Views: 117009

RT-PCR

in such case you will have mixture of random lengths and only the length of amplification step will influence the maximal length of your amplicons

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by JackBean
Fri Feb 01, 2013 11:18 am
 
Forum: Molecular Biology
Topic: RT-PCR
Replies: 9
Views: 4875

Pyrosequencing

Hello, I would like to sequence a gene region with a couple of mutation hot spots nearby each other, and I have a few questions. I guess after PCR amplification of the gene region of interest, I should use a sequencing primer that anneals to a region that has no mutation hot spots. The I generate ...

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by kk
Wed Jan 23, 2013 3:08 pm
 
Forum: Molecular Biology
Topic: Pyrosequencing
Replies: 2
Views: 1750
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