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Dictionary » A » Amplification

Amplification

Definition

noun

(1) The act or result of increasing in size or effect.

(2) An increase in the frequency of a gene or chromosomal region, as a result of replicating a DNA segment by in vivo or in vitro process, such as by gene duplication or polymerase chain reaction, respectively.


Supplement

Word origin: Latin amplificatio < amplificare (“‘to amplify’”).
Related form: amplify (verb)

Related term: gene amplification


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RT-PCR

in such case you will have mixture of random lengths and only the length of amplification step will influence the maximal length of your amplicons

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by JackBean
Fri Feb 01, 2013 11:18 am
 
Forum: Molecular Biology
Topic: RT-PCR
Replies: 9
Views: 3264

Pyrosequencing

Hello, I would like to sequence a gene region with a couple of mutation hot spots nearby each other, and I have a few questions. I guess after PCR amplification of the gene region of interest, I should use a sequencing primer that anneals to a region that has no mutation hot spots. The I generate ...

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by kk
Wed Jan 23, 2013 3:08 pm
 
Forum: Molecular Biology
Topic: Pyrosequencing
Replies: 2
Views: 389

RT-PCR

as far as i know and correct me if i am wrong, RT-PCR amplification products should be short, not too short but not too long, if I understood correctly you are amplifying a 2Kb amplicon I think that is very very long.

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by bravebeaker
Wed Jan 23, 2013 8:24 am
 
Forum: Molecular Biology
Topic: RT-PCR
Replies: 9
Views: 3264

Load DNA PCR Product into Agarose Gel

well, in such case, it could be that you had that band also before, it just wasn't visible. The amplification was not that strong, but after 5 more circles the amount of DNA is 32-times higher.

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by JackBean
Sat Nov 10, 2012 6:37 pm
 
Forum: Molecular Biology
Topic: Load DNA PCR Product into Agarose Gel
Replies: 10
Views: 1166

Mastermix in PCR

... the original sequence. MgCl2 also can stabilize dsDNA structures. Manipulation of MgCl2 concentration (increasing it) is often used to increase amplification of "hard to amplify" DNA samples. However, this increases the amplification of "non-specific" PCR products. PCR products ...

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by daddyjames
Sun Oct 14, 2012 3:43 pm
 
Forum: Bioinformatics
Topic: Mastermix in PCR
Replies: 4
Views: 947
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