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Dictionary » A » Amplification

Amplification

Definition

noun

(1) The act or result of increasing in size or effect.

(2) An increase in the frequency of a gene or chromosomal region, as a result of replicating a DNA segment by in vivo or in vitro process, such as by gene duplication or polymerase chain reaction, respectively.


Supplement

Word origin: Latin amplificatio < amplificare (“‘to amplify’”).
Related form: amplify (verb)

Related term: gene amplification


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Calculating DNA concentration in PCR product

simply by using uv spectrophotometer you can estimate the concentration of dna after pcr amplification

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by shahensha
Sun May 03, 2009 6:26 am
 
Forum: Molecular Biology
Topic: Calculating DNA concentration in PCR product
Replies: 6
Views: 1698

Mutation

... a blank). 1). Point mutation on the gene ..................... 2). Chromosomal rearrangement that involved the gene .................... 3). Gene amplification ..................... 4). Oncogenic retrovirus insertion ..................... What I'm sure enough is number 4 for the gene amplification ...

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by whizzbee
Sun Mar 01, 2009 3:42 pm
 
Forum: Molecular Biology
Topic: Mutation
Replies: 0
Views: 450

rna interference

as I understand the system, the only difference in plants and animals is that in plants there can be an amplification step: after the RNA is exported into the cytoplasm, there can be an amplification of the signal by copying this RNA by an endogenous RNA-dependent RNA polymerase, ...

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by MrMistery
Wed Feb 11, 2009 4:28 am
 
Forum: Molecular Biology
Topic: rna interference
Replies: 4
Views: 722

PCR question

Why is there a need for PCR when there is helicase-dependent amplification?????? In http://en.wikipedia.org/wiki/Helicase-dependent_amplification, wikipedia states that HDA takes longer than PCR. My question is WHY ?????? [Even though the helicase might ...

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by thewax
Tue Dec 23, 2008 1:50 am
 
Forum: Molecular Biology
Topic: PCR question
Replies: 8
Views: 784

dideoxy chain-termination method for sequencing DNA

thewax, That is a legitimate question. You need to understand that in the chain termination method, after doing the amplification in the presence of ddNTPs you separate the molecules by size by electrophoresis. The electrophoresis is sensible enough to separate molecule that differ ...

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by MrMistery
Mon Dec 15, 2008 4:56 am
 
Forum: Molecular Biology
Topic: dideoxy chain-termination method for sequencing DNA
Replies: 9
Views: 1654
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