Ampicillin resistance

... urls, would be appreciated. This experiment was cloning a kanamycin resistance gene into a plasmid and determining the orientation of insert. ... the transformants produced with the ligated DNA were also plated on ampicillin medium. Would you expect to see a significant difference in the ...

See entire post

In short: Ampicillin is active only during cell division. while many other antibiotics are active ... are very likely to kill the bacteria before it even has time to express the antibiotic resistance gene. So the delay is to allow correct expression before trying to poison the ...

See entire post

In short: Ampicillin is active only during cell division. while many other antibiotics are active ... are very likely to kill the bacteria before it even has time to express the antibiotic resistance gene. So the delay is to allow correct expression before trying to poison the ...

See entire post

... the plasmid has enter inside the bacteria and gives it the antibiotic resistance and the presence of the gene to clone (or empty plasmid) , but ... were that have plasmid. At more longer times when betalactamase brokes ampicillin, satellite colonies will apear around the harboring plasmid ones. ...

See entire post

... into bacterial cells. The vector usually has selectable marker such as ampicillin resistance gene that when plated in the presence of ampicillin will survive/produce colonies ...

See entire post