Dictionary » A » Ampicillin resistance

Ampicillin resistance

Ampicillin resistance

Nonsusceptibility of a microbe to the action of ampicillin, a penicillin derivative that interferes with cell wall synthesis.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


cDNA cloning and Lentiviral transfection

... transfection has a bacterial promoter, origin of replication, and Ampicillin antibiotic resistance gene, to allow you to amplify it in bacteria. I don't know what your vector is, ...

See entire post
by BlahBlahBlah
Fri Jul 12, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: cDNA cloning and Lentiviral transfection
Replies: 1
Views: 868

Help Needed - Ligation Pairing (Vector and DNA)

... urls, would be appreciated. This experiment was cloning a kanamycin resistance gene into a plasmid and determining the orientation of insert. ... the transformants produced with the ligated DNA were also plated on ampicillin medium. Would you expect to see a significant difference in the ...

See entire post
by techbrit
Sun Mar 13, 2011 7:34 pm
 
Forum: Molecular Biology
Topic: Help Needed - Ligation Pairing (Vector and DNA)
Replies: 0
Views: 1822

Re:

In short: Ampicillin is active only during cell division. while many other antibiotics are active ... are very likely to kill the bacteria before it even has time to express the antibiotic resistance gene. So the delay is to allow correct expression before trying to poison the ...

See entire post
by poobear
Thu Nov 18, 2010 3:05 pm
 
Forum: Molecular Biology
Topic: Why can you plate directly when having ampicillin?
Replies: 4
Views: 6330

Why can you plate directly when having ampicillin?

In short: Ampicillin is active only during cell division. while many other antibiotics are active ... are very likely to kill the bacteria before it even has time to express the antibiotic resistance gene. So the delay is to allow correct expression before trying to poison the ...

See entire post
by canalon
Thu Nov 18, 2010 1:54 pm
 
Forum: Molecular Biology
Topic: Why can you plate directly when having ampicillin?
Replies: 4
Views: 6330

Re: Why can you plate directly when having ampicillin?

... the plasmid has enter inside the bacteria and gives it the antibiotic resistance and the presence of the gene to clone (or empty plasmid) , but ... were that have plasmid. At more longer times when betalactamase brokes ampicillin, satellite colonies will apear around the harboring plasmid ones. ...

See entire post
by protold
Thu Nov 18, 2010 6:41 am
 
Forum: Molecular Biology
Topic: Why can you plate directly when having ampicillin?
Replies: 4
Views: 6330
View all matching forum results

This page was last modified 21:16, 3 October 2005. This page has been accessed 4,394 times. 
What links here | Related changes | Permanent link