Ampicillin

... long. What might be the cause of this? The only thing I can think might be the problem is her antibiotic being degraded/the wrong concentration? (ampicillin) Any other ideas would be gratefully received

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... the transformation plate. I performed transformation of cloned ANO5 cDNA and the transformation mixture was plated on a LB agar plate containing ampicillin +IPTG+X-Gal. The ANO5 cDNA was cloned in the expression vector pdEYFPC1amp. No blue colonies were detected in any my transformation plates ...

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Hi, so I have been transforming recombinant plasmid DNA into E.coli bacteria to clone the DNA. The plasmid consist of an ampicillin resistant gene. After I grow the bacteria, I would purify the plasmid DNA out of the bacterial cell using a maxi prep kit. So I was wondering if the final ...

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... band as I should..I've picked every positive colony after running a single colony PCR and inoculatedeither 5mL or 10mL of LB-media each time (w/ ampicillin)...the other day I did a 50mL o/n culture and did a miniprep on 30mL ...saw a brighter band than usual (this was after the ethanol precipitation ...

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... to be the # of bands that would form on an agarose gel after electrophoresis of a plasmid which contained one kanamycin gene (1875 bp) and one ampicillin gene (3755 bp), and to explain our prediction. We didn't really learn much about this in class; it's more of an individual learning assignment. ...

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