Alpha

Okay, after doing some reading, I have come up with a different plan for quantifying HIF-1 alpha, which involves running an ELISA after performing a nuclear extraction. However, all the protocols and kits I have seen recommend 1-10 million cells for a good extraction. ...

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Hi, I am trying to quantify HIF 1 alpha protein level. Our scaffold is not a polystyrene plate, and the cells are not grown as 2D monolayers (I am looking at cell aggregates to be specific). Our original plan is to isolate the protein using ...

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... human partner protein called ADP-ribosylation factor 6 (Arf6). [13] Binding exposes its active site, allowing it to permanently ribosylate the Gs alpha subunit of the heterotrimeric G protein. This results in constitutive cAMP production, which in turn leads to secretion of H2O, Na+, K+, Cl−, ...

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hi all How do predict what types of secondary structure (left alpha helices, right alpha helices, parallel beta sheet, anti parallel beta sheet) from amino acid sequence? Reply as soon as possible

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Hi I wonder if someone can tell me what causes black coloration of sheep blood agar caused by bacterial grow? At first I though it was an alpha hemolysis, but I think it is not since it should be yellow and green, not black. the black coloration I have observed looked like that: http://microculture.tumblr.com/post/1665565422/bacillus-anthracis-this-image-depicted-numbers-of ...

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