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Alkaline d-peptidase

Alkaline d-peptidase

(Science: enzyme) a penicillin-recognizing enzyme from bacillus cereus; has beta-lactamase activity; genbank d86380

registry number: EC 3.4.99.-

Synonym: adp gene product, alkaline d-stereospecific endopeptidase

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Conflicting results of Gram-Negative unknown

... no growth on the slant 8. Motility test *Negative for motility No red spreading throughout media FLOW CHART looks something like this TSI SLANT (ALKALINE/ACID) = Lactose Negative Sulfer reduction of TSI slant= Negative then must be Urease NEGATIVE * I am sure the unknown is negative for sulfer ...

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by Jrh21854
Sun Dec 01, 2013 8:25 pm
Forum: Microbiology
Topic: Conflicting results of Gram-Negative unknown
Replies: 0
Views: 3626

ligation problem although try everything,suggestions?

... said (NEB)alternatively try 25 minutes and also tried T4 DNA ligase at room temperature (NEB). I make 1:1, 1:3, 1:5 and 1:10 ligations. I make alkaline phosphatase and then try ligation again. I used 50 ng vector,15 ng insert it did not worked. I used 100 ng vector, it also not worked. I used ...

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by genetherapy
Wed May 16, 2012 3:31 pm
Forum: Molecular Biology
Topic: ligation problem although try everything,suggestions?
Replies: 7
Views: 5459

Help identifying unknown bacteria

... deep stab slow urea hydrolysis, weak urease prod-urea agar citrate utilization-citrate test no glucose fermentation, degradation of peptone and alkaline end products with sucrose and lactose fermentation test-Phenol Red tests EMB test- gram negative SIM test- tryptophan broken down, indole positive, ...

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by anthony7
Sun Apr 01, 2012 9:21 pm
Forum: Microbiology
Topic: Help identifying unknown bacteria
Replies: 8
Views: 5873

Re: Odd food poisoning-like symptoms, strange tastes

... wake up in the middle of the night with the stabbing pains you all have ! drinking water only neutralizes the ammonia fast relief can come from alkaline foods like sea-weeds spirulina, wheat grass etc. i would advise hot lemon tea however must of you have scraped your insides and lemon is Acidic ...

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by ElieSiag
Wed Feb 29, 2012 9:31 am
Forum: Human Biology
Topic: Odd food poisoning-like symptoms, strange tastes
Replies: 35
Views: 136845

What bacteria does this TSI test indicate?

... result. I couldn't find anything like it in the book or online. Any help and further explanation would really be appreciated :) TSI had red butt (alkaline) and black (H2s) on slant. There was no acid (or yellow) production. However, I've read online that the H2S can sometimes mask that so I'm ...

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by SweetSarah
Mon Feb 27, 2012 5:46 am
Forum: Microbiology
Topic: What bacteria does this TSI test indicate?
Replies: 5
Views: 5128
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