Alkaline

... said (NEB)alternatively try 25 minutes and also tried T4 DNA ligase at room temperature (NEB). I make 1:1, 1:3, 1:5 and 1:10 ligations. I make alkaline phosphatase and then try ligation again. I used 50 ng vector,15 ng insert it did not worked. I used 100 ng vector, it also not worked. I used ...

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... deep stab slow urea hydrolysis, weak urease prod-urea agar citrate utilization-citrate test no glucose fermentation, degradation of peptone and alkaline end products with sucrose and lactose fermentation test-Phenol Red tests EMB test- gram negative SIM test- tryptophan broken down, indole positive, ...

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... wake up in the middle of the night with the stabbing pains you all have ! drinking water only neutralizes the ammonia fast relief can come from alkaline foods like sea-weeds spirulina, wheat grass etc. i would advise hot lemon tea however must of you have scraped your insides and lemon is Acidic ...

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... result. I couldn't find anything like it in the book or online. Any help and further explanation would really be appreciated :) TSI had red butt (alkaline) and black (H2s) on slant. There was no acid (or yellow) production. However, I've read online that the H2S can sometimes mask that so I'm ...

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... look like any of the examples in my lab book nor could I find anything like this online. I'm not sure if I should redo it. My TSI slant has a red (alkaline) butt but has black on top, which I'm assuming is for H2S (hydrogen sulfide) production. But what confuses me is that there's no acid production ...

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