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Dictionary » A » Aliquot

Aliquot

Definition

adjective

Of, relating to or denoting an exact factor or divisor of a quantity, i.e. not resulting in a remainder, e.g. an aliquot part of 8 is 2.

noun

(mathematics) An aliquot part; a portion of a whole.

(chemistry) A sample or a portion of the total amount of a solution.

(pharmacology) A method of measuring ingredient below the sensitivity of a scale.


Supplement

In chemistry and pharmacology, it is common that a solution of a reagent is divided into aliquots so that there is no need in handling the total sample when a small portion is only needed.


Word origin: from the Latin aliquoties, "several times"

Compare: aliquant


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Problems making chemically competent cells

... step 5. 9. Resuspend the pellet in 8ml (1/12.5 of the original culture volume) of RF2. 10. Incubate the cell suspension on ice for 15 minutes. 11. Aliquot the cells into pre-chilled eppendorfs. 12. (Immediately flash freeze the aliquots in liquid nitrogen, then store at -70°C.) Solutions: RF1 Compound ...

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by lilruthc
Fri Feb 08, 2013 1:09 pm
 
Forum: Molecular Biology
Topic: Problems making chemically competent cells
Replies: 1
Views: 352

Re: Bacterial Calculation

... need a serial dilution calculator ... seriously. If you get 55 colonies at a 10^4 dilution, then you have 5.5*10^5 cfu/mL (in case you used an aliquot of 1 mL). If you count 85 colonies at a 10^5 dilution then it`s 8.5*10^6 cfu/mL, which is already close to the concentration you need. To get ...

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by EnricoPallazzo
Sun Jan 13, 2013 9:43 am
 
Forum: Microbiology
Topic: Bacterial Calculation
Replies: 3
Views: 678

Resuspending bacterial pellet to a certain OD600

What about to get only aliquot of your O/N culture? :roll:

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by JackBean
Fri Jun 29, 2012 7:12 am
 
Forum: General Discussion
Topic: Resuspending bacterial pellet to a certain OD600
Replies: 8
Views: 1534

Help growing Chlamydia stock

... trying to culture Chlamydia (C. pecorum and C. trachomatis, in BGM cells), and have never done any cell culture work before. I have a few 100uL aliquots of C. trachomatis stock that I need to grow up in order to make 100's of aliquots, to use as my positive control. Each aliquot is apparently ...

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by bluedory
Fri Jun 29, 2012 2:47 am
 
Forum: Microbiology
Topic: Help growing Chlamydia stock
Replies: 0
Views: 751

How to extract lactic acid bacteria without nutrient broth?

... be abundant, only say 250 ul could work. But I'm not able to provide real answer for this. However, from what I remember from our micro lab, only aliquot should work, if you want dilute it too much (say you will use 1 volume unit per 1 mass unit, i. e. 1 ml of your solution per 1 g of natto)

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by JackBean
Fri Feb 10, 2012 7:37 am
 
Forum: Microbiology
Topic: How to extract lactic acid bacteria without nutrient broth?
Replies: 11
Views: 2278
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