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Dictionary » A » Aliquot AliquotDefinition adjective Of, relating to or denoting an exact factor or divisor of a quantity, i.e. not resulting in a remainder, e.g. an aliquot part of 8 is 2. noun (mathematics) An aliquot part; a portion of a whole. (chemistry) A sample or a portion of the total amount of a solution. (pharmacology) A method of measuring ingredient below the sensitivity of a scale.
In chemistry and pharmacology, it is common that a solution of a reagent is divided into aliquots so that there is no need in handling the total sample when a small portion is only needed.
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Results from our forumRe: How to be "good at the bench"... and if you need to put down, put it lid down 12. Have your own buffer stocks for restriction enzymes, PCRs etc. Don’t use the common stock. 13. Aliquot out everything, from primers to buffers to enzymes. In case of suspected contamination, you can always discard one tube and check. 14. Working ...
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Radioactivity Q... 6ul 5x tailing buffer 2ul Alu I cut lambda DNA 2ul 1mM dCTP 2ul 32p DTP 1ul (15U) of TdT The reaction mix was incubated at 37C and after 5min, 2ul aliquots were removed from the reaction mix to determine the amount of radioactivity incorporated. One aliquot was counted directly to determine the ...
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Re: Rapid testing techniques - food... tests. Here is a link to a product description. http://www2.dupont.com/Qualicon/en_US/products/Lateral_Flow_System/lfs_ecoli.html You would add an aliquot of the incubated media. The test containes antibodies on it for a the E.coli RFB protien and it will produce a red line if it is present During ...
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Porblems making DIG probe - please help... The reason I ask is that someone in our lab was spinning it down (!) and drawing off the supernatant to use in her reactions, ruining the whole aliquot. When it comes to retardation, the perpetrator is never the only victim ;) Ask other lab members what they do to find out if they're sabotaging ...
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plasmid DNA questionUsually, you make (or buy) competent cells in advance and store them at -70. You thaw a small aliquot on ice just before using. If your stored cells are competent, that should be all you need -- well, that and your plasmid -- and you should be in business. Surely your ...
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