Air chamber

... before, many fish ( Corydoras catfish, for example) can swallow air from the water's surface and absorb oxygen through their intestinal lining. Lungs evolved as a chamber of the intestine that gradually split off into its own organ. In essence, your ...

See entire post

... before, many fish ( Corydoras catfish, for example) can swallow air from the water's surface and absorb oxygen through their intestinal lining. Lungs evolved as a chamber of the intestine that gradually split off into its own organ. In essence, your ...

See entire post

... 200 because the tip is much narrower. If all else fails, that might solve your problem. 4. If the clumps are small, leave them as is. The counting chamber has a huge error anyway. 5. I just thought of this, no idea if it would work: what about putting the cells in a Corning tube and vortexing them? ...

See entire post

... PMA instead of PHA, but as these twomitogens are pretty similar, I don't think this make a huge difference. I am counting my cells on a Neubauer chamber with trypan blue, and I am also using a nucleocounter to get the number of cells/their viability. I wasn't aware of the CFSE light sensitivity. ...

See entire post

... can spoil it. Btw, what methods you use to ensure that your cells have prolified? Do you just check them under a microscope or count them in a chamber, or just look at the colour of your medium? What cells do you use? For example T cells need proper stimulants to make them divide (e.g. PHA ...

See entire post