Dictionary » A » Aggregates



(Science: zoology) a group of species, other than a subgenus, within a genus, or a group of subspecies within a species. An aggregate may be denoted by a group name interpolated in parentheses.

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... MMPs are spherical, although some are ovoid in morphology, and all appear to possess a central, acellular compartment. The MMP divides as aggregates without an individual cell stage. Phylogenetic analysis and phenotypic characterization were used to assign a multicellular magnetotactic ...

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by BlahBlahBlah
Thu Jun 27, 2013 1:46 pm
Forum: Microbiology
Topic: Multicellular prokaryote
Replies: 27
Views: 28004

Protein isolation

... to quantify HIF 1 alpha protein level. Our scaffold is not a polystyrene plate, and the cells are not grown as 2D monolayers (I am looking at cell aggregates to be specific). Our original plan is to isolate the protein using trizol extraction reagent, then run an ELISA on HIF 1 alpha. However, ...

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by roth317
Tue Feb 12, 2013 9:25 pm
Forum: Molecular Biology
Topic: Protein isolation
Replies: 2
Views: 2078

Did not dilute Lipofectamine during Transfection !?

... forgot to dilute the Lipofectamine before I mixed the contents after waiting the 5 minutes. Will it still work ??? I know that Lipofectamine forms aggregates but is it really that bad to not dilute the Lipofectamine ?

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by Hicsuntdrac0nis
Thu Sep 06, 2012 11:49 pm
Forum: Molecular Biology
Topic: Did not dilute Lipofectamine during Transfection !?
Replies: 0
Views: 1610

Re: glass cuvette cleaning

... bacterial turbidities, for which some trace contamination on the glassware is unlikely to skew the measurement. If you are measuring chemical aggregates which might be flocculated or disrupted by chemicals in the pipet, more rigorous cleaning might be appropriate to avoid skewing the turbidity ...

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by jonmoulton
Tue Mar 06, 2012 4:50 pm
Forum: General Discussion
Topic: glass cuvette cleaning
Replies: 2
Views: 3394

Removing MBP tag

And, I already know that my protein highly aggregates. DLS measurement showed huge but stable aggregates which surprised us. This would explain why it is so hard to cut it completely or why so difficult to perform gel filtration (always found ...

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by Papaver
Fri Oct 21, 2011 11:58 am
Forum: Molecular Biology
Topic: Removing MBP tag
Replies: 12
Views: 8853
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