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Agarose gel electrophoresis

Agarose gel electrophoresis

(Science: procedure) a type of electrophoresis that uses a matrix of highly purified agar to separate large nucleotides in size.


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How to prepare a 3.5% agarose gel ?

I tried a prepare a 3.5% agarose gel for electrophoresis, i tried to cook it on a hot plate with a rotating magnet, ...

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by itamo
Mon Feb 11, 2013 1:32 pm
 
Forum: Molecular Biology
Topic: How to prepare a 3.5% agarose gel ?
Replies: 4
Views: 949

restriction analysis

... You can purify the product (your gene) from agarose gel or use a PCR purification kit (Qiagen for example). ... transform to TOP10 and you are done. For Agarose gel electrophoresis check: http://en.wikipedia.org/wiki/Gel_electrophoresis ...

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by citroenboom
Tue Jul 17, 2012 7:10 am
 
Forum: Molecular Biology
Topic: restriction analysis
Replies: 14
Views: 4282

Electrophoresis conditions

Hi all, I use 35 centimetre agarose gel tank, we use constant watt (20 watt), change only the mA and voltage. I set voltage to 140V and mA to ...

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by genetherapy
Wed Mar 14, 2012 1:53 pm
 
Forum: Molecular Biology
Topic: Electrophoresis conditions
Replies: 0
Views: 453

Re:

1st make sure you are using right agarose. I recommend using this one at 2% for best ... Gel+Materials%23rpp%3D15&fromSearch=1&searchKey=agarose&highlightProductsItemsFlag=Y ... but using Low melting agarose for regular electrophoresis is a waste of money. The OP has not ...

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by canalon
Tue Nov 08, 2011 1:55 am
 
Forum: Genetics
Topic: DNA Gel Electrophoresis Issues - Bad Ladder Separation
Replies: 4
Views: 4848

questions

... according with his sizes when subjected to electrophoresis in gel how we visualize the dna after the electrophoresis in gel of agarose? what is the use of TAE solution and what results can we ...

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by Avicii5
Sat Nov 05, 2011 11:30 pm
 
Forum: Cell Biology
Topic: questions
Replies: 2
Views: 537
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