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Agarose gel electrophoresis

Agarose gel electrophoresis

(Science: procedure) a type of electrophoresis that uses a matrix of highly purified agar to separate large nucleotides in size.


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Asking for help with electrophoresis

Thanks for all of your help! I did more gel electrophoresis and got quite good results this time. Below was what I changed: 1. 1.8% agarose gel, but still regular agarose 2. When melting the agarose, ...

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by ll2013
Mon Jun 17, 2013 1:51 pm
 
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 2142

Asking for help with electrophoresis

Dear all, my lab has some problems with DNA agarose gel electrophoresis. First, the small DNAs cannot be separated. We are ...

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by ll2013
Thu Jun 06, 2013 1:09 am
 
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 2142

How to prepare a 3.5% agarose gel ?

I tried a prepare a 3.5% agarose gel for electrophoresis, i tried to cook it on a hot plate with a rotating magnet, ...

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by itamo
Mon Feb 11, 2013 1:32 pm
 
Forum: Molecular Biology
Topic: How to prepare a 3.5% agarose gel ?
Replies: 4
Views: 2123

restriction analysis

... You can purify the product (your gene) from agarose gel or use a PCR purification kit (Qiagen for example). ... transform to TOP10 and you are done. For Agarose gel electrophoresis check: http://en.wikipedia.org/wiki/Gel_electrophoresis ...

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by citroenboom
Tue Jul 17, 2012 7:10 am
 
Forum: Molecular Biology
Topic: restriction analysis
Replies: 14
Views: 7196

Electrophoresis conditions

Hi all, I use 35 centimetre agarose gel tank, we use constant watt (20 watt), change only the mA and voltage. I set voltage to 140V and mA to ...

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by genetherapy
Wed Mar 14, 2012 1:53 pm
 
Forum: Molecular Biology
Topic: Electrophoresis conditions
Replies: 0
Views: 710
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