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Agarose gel electrophoresis

Agarose gel electrophoresis

(Science: procedure) a type of electrophoresis that uses a matrix of highly purified agar to separate large nucleotides in size.


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Asking for help with electrophoresis

Thanks for all of your help! I did more gel electrophoresis and got quite good results this time. Below was what I changed: 1. 1.8% agarose gel, but still regular agarose 2. When melting the agarose, ...

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by ll2013
Mon Jun 17, 2013 1:51 pm
 
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 2348

Asking for help with electrophoresis

Dear all, my lab has some problems with DNA agarose gel electrophoresis. First, the small DNAs cannot be separated. We are ...

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by ll2013
Thu Jun 06, 2013 1:09 am
 
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 2348

Campylobacter RFLP troubleshooting

... to the procedure suggested by Fermentas. I am using % 4 Nusieve GTG agarose and 100 bp ladder between 100-1000 bp during electrophoresis and rUn the digests at 90 V for 90 minutes. After staining with etidium broimde, ...

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by gislab03
Sat May 04, 2013 1:59 pm
 
Forum: Molecular Biology
Topic: Campylobacter RFLP troubleshooting
Replies: 4
Views: 2077

How to prepare a 3.5% agarose gel ?

I tried a prepare a 3.5% agarose gel for electrophoresis, i tried to cook it on a hot plate with a rotating magnet, ...

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by itamo
Mon Feb 11, 2013 1:32 pm
 
Forum: Molecular Biology
Topic: How to prepare a 3.5% agarose gel ?
Replies: 4
Views: 2278

pcr purification trouble

... If you want to check your PCR, you have to use either fluorescence or agarose electrophoresis. Sorry, I didn't get the other thing. But if you want to calculate mass of ...

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by JackBean
Sun Aug 12, 2012 8:38 pm
 
Forum: Molecular Biology
Topic: pcr purification trouble
Replies: 4
Views: 3954
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