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Agarose

Agarose

(Science: chemical) this linear galactan is created by purifying agar, when it is heated and cooled, it forms a gel that is used as a support for many types of electrophoresis and immunodiffusion (agarbiose). a typical gel is about 1% agarose.

agarose is more porous than acrylamide and is sold in different grades, the lower its sulphate content, the more highly purified it is.


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Basic Knowledge about DNA Extraction

... off and the DNA will be found at the interface between the two phases. Finally, the presence of DNA can be confirmed by electrophoresing on an agarose gel or another fluorescent dye that reacts with the DNA, and also by UV light. Lab instruments used in DNA Extraction Bead beater is often used ...

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by 2444256703
Thu Jan 22, 2015 8:56 am
 
Forum: Genetics
Topic: Basic Knowledge about DNA Extraction
Replies: 0
Views: 749

separation problem with GelRed

... the usual amount of DNA. 2. Reduce the amount of GelRed. Try using 0.5X instead of 1X final concentration of GelRed. 3. Pour a lower percentage of agarose gel. Higher molecular weight DNA separates better with a lower percentage gel. 4. Change the running buffer. TBE buffer has a higher buffering ...

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by Biotium
Wed Nov 26, 2014 4:43 pm
 
Forum: Molecular Biology
Topic: separation problem with GelRed
Replies: 12
Views: 19558

plasmid extraction on 1.5% agarose gel

So I have two bands, the smaller that is around 8-9kb based on the marker ( my plasmids should be 8-9 kb), what is the upper band? Also I have three plasmids and I did this in a set of four replicates

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by cah2010
Wed Dec 18, 2013 2:23 am
 
Forum: Molecular Biology
Topic: plasmid extraction on 1.5% agarose gel
Replies: 5
Views: 2726

Asking for help with electrophoresis

Thanks for all of your help! I did more gel electrophoresis and got quite good results this time. Below was what I changed: 1. 1.8% agarose gel, but still regular agarose 2. When melting the agarose, instead of using microwave, this time I used a heating block with stirring. 3. Used a much ...

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by ll2013
Mon Jun 17, 2013 1:51 pm
 
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 3339

Asking for help with electrophoresis

Good resolution of the bands over 1kb on the gel in the picture tells me that regular agarose was used - resolving power of that is usually 200bp and up. Increasing the percentage of agarose would improve resolution of smaller fragments somewhat, but if GOOD resolution ...

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by Cat
Sun Jun 09, 2013 4:47 pm
 
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 3339
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