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Agarose

Agarose

(Science: chemical) this linear galactan is created by purifying agar, when it is heated and cooled, it forms a gel that is used as a support for many types of electrophoresis and immunodiffusion (agarbiose). a typical gel is about 1% agarose.

agarose is more porous than acrylamide and is sold in different grades, the lower its sulphate content, the more highly purified it is.


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Re: PCR & Agarose gel electrophoresis

Hi! Could you describe the experiment more detailed? where do the primers anneal? why are there two bands? did you use more than 2 primers? which polymerase did you use (proofreading or not)? does a primer anneal to the mutated site? I don't think that contamination is the explanation to that. I'd e...

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by apolll
Sun Aug 23, 2009 8:40 pm
 
Forum: Molecular Biology
Topic: PCR & Agarose gel electrophoresis
Replies: 3
Views: 122

PCR & Agarose gel electrophoresis

... my particular experiment went wrong or what could have been the reasons. Can anyone help me as to what kind of things can go wrong with PCR and agarose gel electrophoresis experiments. The only thing I can think of is contamination of DNA samples but there must be other reasons. I would be grateful ...

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by Juanita1
Thu Aug 20, 2009 8:21 pm
 
Forum: Molecular Biology
Topic: PCR & Agarose gel electrophoresis
Replies: 3
Views: 122

Western Blot from an Agarose gel...?

I've never heard of anyone running proteins through an agarose gel before (why are you doing it btw?). There isn't any mechanistic aspect to blotting the gel (except maybe that it is too thick to fit into a western transfer machine), but I think you ...

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by MrMistery
Fri Aug 14, 2009 6:20 pm
 
Forum: General Discussion
Topic: Western Blot from an Agarose gel...?
Replies: 3
Views: 97

Western Blot from an Agarose gel...?

Hello all, i made a 4% agarose gel for protein separation using 1x tgs buffer. I was wondering if it would be pointless (or even detrimental) to perform a Western blot on the same gel, just to visualize it better. help? Thanks

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by herm913
Thu Aug 13, 2009 12:56 pm
 
Forum: General Discussion
Topic: Western Blot from an Agarose gel...?
Replies: 3
Views: 97

G genes

... the following things: - Protein G genes - Recombinant His-tagged proteins (and how they are purified by affinity chromatography on a nickel-agarose column) Thank you very much for your help! - Tim

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by zhetimxu
Sun Jun 28, 2009 7:21 pm
 
Forum: General Discussion
Topic: G genes
Replies: 1
Views: 58
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