Dictionary » A » Agarose

Agarose

Agarose

(Science: chemical) this linear galactan is created by purifying agar, when it is heated and cooled, it forms a gel that is used as a support for many types of electrophoresis and immunodiffusion (agarbiose). a typical gel is about 1% agarose.

agarose is more porous than acrylamide and is sold in different grades, the lower its sulphate content, the more highly purified it is.


Please contribute to this project, if you have more information about this term feel free to edit this page



Results from our forum


plasmid extraction on 1.5% agarose gel

So I have two bands, the smaller that is around 8-9kb based on the marker ( my plasmids should be 8-9 kb), what is the upper band? Also I have three plasmids and I did this in a set of four replicates

See entire post
by cah2010
Wed Dec 18, 2013 2:23 am
 
Forum: Molecular Biology
Topic: plasmid extraction on 1.5% agarose gel
Replies: 5
Views: 1583

Asking for help with electrophoresis

Thanks for all of your help! I did more gel electrophoresis and got quite good results this time. Below was what I changed: 1. 1.8% agarose gel, but still regular agarose 2. When melting the agarose, instead of using microwave, this time I used a heating block with stirring. 3. Used a much ...

See entire post
by ll2013
Mon Jun 17, 2013 1:51 pm
 
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 2141

Asking for help with electrophoresis

Good resolution of the bands over 1kb on the gel in the picture tells me that regular agarose was used - resolving power of that is usually 200bp and up. Increasing the percentage of agarose would improve resolution of smaller fragments somewhat, but if GOOD resolution ...

See entire post
by Cat
Sun Jun 09, 2013 4:47 pm
 
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 2141

Asking for help with electrophoresis

ll2013 doesn't need any special agarose, just higher percentage, if wants to see bands 50-300 bp. But than will have problems with the bigger bands.

See entire post
by JackBean
Sat Jun 08, 2013 4:45 pm
 
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 2141

Asking for help with electrophoresis

1. It seems that you are using regular agarose which separates 200bp and up. You need genetic analysis grade agarose that allows good separation of fragments bellow 1kb. And even with that, you will need to make a 2-3% agarose gel. Here is the ...

See entire post
by Cat
Sat Jun 08, 2013 3:57 pm
 
Forum: Molecular Biology
Topic: Asking for help with electrophoresis
Replies: 5
Views: 2141
View all matching forum results

This page was last modified 02:45, 11 April 2007. This page has been accessed 16,674 times. 
What links here | Related changes | Permanent link