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Agarose

Agarose

(Science: chemical) this linear galactan is created by purifying agar, when it is heated and cooled, it forms a gel that is used as a support for many types of electrophoresis and immunodiffusion (agarbiose). a typical gel is about 1% agarose.

agarose is more porous than acrylamide and is sold in different grades, the lower its sulphate content, the more highly purified it is.


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How to prepare a 3.5% agarose gel ?

... do it in the bottle with cap and I keep it on (just not tighten it firmly). As long as it is not properly dissolved. I'm usually working with 1% agarose, but I think I have prepared also 4% in this way.

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by JackBean
Tue Feb 12, 2013 10:10 am
 
Forum: Molecular Biology
Topic: How to prepare a 3.5% agarose gel ?
Replies: 4
Views: 374

How to make high concentration agarose gel

Thanks. Do you cover the flask to avoid evaporation ? If you do, with what ? Did you tried it yourself? How many cycles of heating and swirling did you do?

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by itamo
Tue Feb 12, 2013 6:43 am
 
Forum: Molecular Biology
Topic: How to prepare a 3.5% agarose gel ?
Replies: 4
Views: 374

How to prepare a 3.5% agarose gel ?

I tried a prepare a 3.5% agarose gel for electrophoresis, i tried to cook it on a hot plate with a rotating magnet, but there were alot of bubbles in the gel. Is there a way to prepare a high concentration gel without the annoying bubbles?

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by itamo
Mon Feb 11, 2013 1:32 pm
 
Forum: Molecular Biology
Topic: How to prepare a 3.5% agarose gel ?
Replies: 4
Views: 374

Basics for preparing bacterial cultures

... However, I would disagree about the microwave, we use it standardly for melting agarose and agar should work as well. One just has to check that it doesn't boil. It's always good to mix the bottle during heating. By that it melts ...

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by JackBean
Sun Nov 25, 2012 11:13 pm
 
Forum: Microbiology
Topic: Basics for preparing bacterial cultures
Replies: 4
Views: 953

Load DNA PCR Product into Agarose Gel

... without changing any things except I increased No. of cycling to 35 instead of 30 so is that a big deal and may appear as a double band in Agarose Gel ???

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by 9afa
Sat Nov 10, 2012 6:09 pm
 
Forum: Molecular Biology
Topic: Load DNA PCR Product into Agarose Gel
Replies: 10
Views: 1166
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