Affinity column

Affinity column --> affinity chromatography

(Science: investigation) a technique of analytical chemistry used to separate and purify a biological molecule from a mixture, based on the attraction of the molecule of interest to a particular ligand which has been previously attached to a solid, inert substance.

The mixture is passed through a column containing the ligand attached to the stationary substance, so that the molecule of interest stays within the column while the rest of the mixture continues through to the end. Then, a different chemical is flushed through the column to detach the molecule from the ligand and bring it out separately from the rest of the mixture.


Please contribute to this project, if you have more information about this term feel free to edit this page



This page was last modified on 3 October 2005, at 21:16. This page has been accessed 2,925 times. 
What links here | Related changes | Permanent link