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Dictionary » A » Affinity column

Affinity column

Affinity column --> affinity chromatography

(Science: investigation) a technique of analytical chemistry used to separate and purify a biological molecule from a mixture, based on the attraction of the molecule of interest to a particular ligand which has been previously attached to a solid, inert substance.

The mixture is passed through a column containing the ligand attached to the stationary substance, so that the molecule of interest stays within the column while the rest of the mixture continues through to the end. Then, a different chemical is flushed through the column to detach the molecule from the ligand and bring it out separately from the rest of the mixture.


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Idea to determine order of assembly of spliceosome

... be able to discriminate your transcript. But you could purify it on affinity column. However, that would be quite laborious. Better would be probably to induce your RNA ...

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by JackBean
Tue May 29, 2012 11:25 am
 
Forum: Molecular Biology
Topic: Idea to determine order of assembly of spliceosome
Replies: 15
Views: 5978

Why dialyse DMEM before protein purification (Ni-Affinity)?

... Ni-NTA Agarose was added and finally these beads where put on a column. Some washing procedures followed (his-dialysis-buffer with either ... is in DMEM that would make it difficult (impossible?) to run the ni-affinity chromatography? Would the protein precipitate? If yes, why then? ...

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by LittleBird
Sun May 20, 2012 3:43 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 2043

Re: T7 Expression System

... system with T7 RNAP then run the blue-sephadex affinity column but also wanted to establish an in-vivo method to try. I've noticed here: http://www.sciencedirect.com/science/article/pii/0014579383806243 ...

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by TjCooper
Tue Jan 03, 2012 1:22 pm
 
Forum: Molecular Biology
Topic: T7 Expression System
Replies: 5
Views: 2981

Re: T7 Expression System

... usable purification tag. I guess I will run it through a blue-sephadex affinity column (as it's a putative adenylate kinase) and see where it goes from there!

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by TjCooper
Tue Jan 03, 2012 11:55 am
 
Forum: Molecular Biology
Topic: T7 Expression System
Replies: 5
Views: 2981

Binding proteins to cytoplasmic surface of cellular membrane

... its suggested that you pass the preparation of mixed vesicles over an affinity column. There is an affinity column made of the lectin pisum sativum from the common pea attached ...

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by havery01
Mon Nov 22, 2010 9:00 pm
 
Forum: Cell Biology
Topic: Binding proteins to cytoplasmic surface of cellular membrane
Replies: 5
Views: 4183
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