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Affinity chromatography

Affinity chromatography

(Science: investigation) a technique of analytical chemistry used to separate and purify a biological molecule from a mixture, based on the attraction of the molecule of interest to a particular ligand which has been previously attached to a solid, inert substance.

The mixture is passed through a column containing the ligand attached to the stationary substance, so that the molecule of interest stays within the column while the rest of the mixture continues through to the end. Then, a different chemical is flushed through the column to detach the molecule from the ligand and bring it out separately from the rest of the mixture.


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Why dialyse DMEM before protein purification (Ni-Affinity)?

... is in DMEM that would make it difficult (impossible?) to run the ni-affinity chromatography? Would the protein precipitate? If yes, why then? I've been using google for ...

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by LittleBird
Sun May 20, 2012 3:43 pm
 
Forum: Molecular Biology
Topic: Why dialyse DMEM before protein purification (Ni-Affinity)?
Replies: 5
Views: 2611

Re: thin layer chromatography

Hi Jack, I agree, though the xanthophylls might move a little. pi clouds might give some affinity with the conjugated mobile phase (benzene). - Jon

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by jonmoulton
Mon Nov 28, 2011 4:59 pm
 
Forum: Molecular Biology
Topic: thin layer chromatography
Replies: 4
Views: 2116

Single stranded DNA

... for some time... Or you could try to digest normal DNA and then do some affinity chromatography specific for only one strand. But I don't know, whether you could get only one ...

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by JackBean
Tue Feb 22, 2011 5:54 pm
 
Forum: Genetics
Topic: Single stranded DNA
Replies: 5
Views: 1969

Re:

... Anyway, you can try what jonmoulton suggested or some kind of affinity chromatography or anything I'm sorry I don't understand "ChIP-chip is for protein-protein ...

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by redhawk
Wed Feb 10, 2010 10:44 pm
 
Forum: Genetics
Topic: How to find the mechanism on differential gene expression
Replies: 7
Views: 3835

How to find the mechanism on differential gene expression

... Anyway, you can try what jonmoulton suggested or some kind of affinity chromatography or anything

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by JackBean
Wed Feb 10, 2010 9:22 pm
 
Forum: Genetics
Topic: How to find the mechanism on differential gene expression
Replies: 7
Views: 3835
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