Dictionary » A » Aeration



(Science: physiology) to add air or oxygen into a liquid.

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Problems making chemically competent cells

... medium 2. The next day, inoculate 100ml pre-warmed SOC medium with 5ml of the overnight pre-culture. The volume of the flask here is important for aeration. I use a 2 litre baffled flask for improved aeration (volume of medium 1/10 or 1/30 volume of the flask). 3. Incubate the culture at 37°C with ...

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by lilruthc
Fri Feb 08, 2013 1:09 pm
Forum: Molecular Biology
Topic: Problems making chemically competent cells
Replies: 1
Views: 3679

Culturing cyanobacteria - urgent advice please!!

... at a ratio of 1:10 with UTEX medium as advised by CCAP. I was not sure on light levels so I put them in a slightly shaded window with very gentle aeration. After two weeks I placed them on a light bench with around 5000 lux of standard white fluorescent light (this as the only value I could find ...

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Wed Jun 20, 2012 10:14 am
Forum: Microbiology
Topic: Culturing cyanobacteria - urgent advice please!!
Replies: 3
Views: 2676

It is going to kill me

... present in soil on top of your carbon source to grow (iron, maybe as heme; Nitrogen source; whatever else) 3- You are working with soil bacteria, aeration is not continuous and even in surface soil there are plenty of nearly anaerobic pockets in the soil. I would also try anaerobic and microanaerobic ...

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by canalon
Thu Jul 07, 2011 4:26 pm
Forum: Microbiology
Topic: It is going to kill me
Replies: 7
Views: 3160

Growth Curve

... phase? How long in Stationary phase? C] From graph determine the mean generation time and then determine the growth rate constant. D]If aeration was interrupted at 120min, draw on the graph what you would expect to observe for next 3 hours. My main problem is drawing the graph. I have ...

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by jackcreambun
Sun Apr 17, 2011 10:12 pm
Forum: Microbiology
Topic: Growth Curve
Replies: 3
Views: 5217

Biology Assignment: Help

... levels =more autotrophs present And I think you should use data from all of them for comparision. pS: R you considering other factors such as aeration?

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by Linn
Sun Mar 29, 2009 2:32 pm
Forum: Ecology
Topic: Biology Assignment: Help
Replies: 1
Views: 8478
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