Aeration

... medium 2. The next day, inoculate 100ml pre-warmed SOC medium with 5ml of the overnight pre-culture. The volume of the flask here is important for aeration. I use a 2 litre baffled flask for improved aeration (volume of medium 1/10 or 1/30 volume of the flask). 3. Incubate the culture at 37°C with ...

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... at a ratio of 1:10 with UTEX medium as advised by CCAP. I was not sure on light levels so I put them in a slightly shaded window with very gentle aeration. After two weeks I placed them on a light bench with around 5000 lux of standard white fluorescent light (this as the only value I could find ...

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... present in soil on top of your carbon source to grow (iron, maybe as heme; Nitrogen source; whatever else) 3- You are working with soil bacteria, aeration is not continuous and even in surface soil there are plenty of nearly anaerobic pockets in the soil. I would also try anaerobic and microanaerobic ...

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... phase? How long in Stationary phase? C] From graph determine the mean generation time and then determine the growth rate constant. D]If aeration was interrupted at 120min, draw on the graph what you would expect to observe for next 3 hours. My main problem is drawing the graph. I have ...

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... levels =more autotrophs present And I think you should use data from all of them for comparision. pS: R you considering other factors such as aeration?

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