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Adjuvant

Adjuvant

1. (Science: pharmacology) a substance that, when added to a medicine, speeds or improves its action which aids another, such as an auxiliary remedy

2. (Science: immunology) a substance added to a vaccine to improve the immune response so that less vaccine is needed to produce a non-specific stimulator (for example, BCG vaccine) of the immune response.

Origin: L. Adjuvans = aiding Relating to something that is added but is not essential; an ancillary pump; an adjuvant discipline to forms of mysticism; The mind and emotions are auxilliary to each other.Enhancing the action of a medical treatment; the adjuvant action of certain bacteria.An additive that enhances the effectiveness of medical treatment.A modified substance that is intended to produce a desirable response. Adjuvants are implanted in viruses to weaken or kill them so that they can be used in immunisation so that the body can code for the respective antibodies in case of future infection.


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Re: What else can I do with my PCR?

... 50 ng of the entire genome. You need to increase your odds a little. I would just do a very basic PCR program at one annealing temp, maybe one adjuvant like <10% DMSO, and vary template: 50, 100, 200, 300 ng and see if this helps.

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by clevermizo
Tue May 25, 2010 9:44 pm
 
Forum: Molecular Biology
Topic: What else can I do with my PCR?
Replies: 8
Views: 2615

nested pcr

I had the same problem before. I advice you to use an adjuvant to improve your PCR effeciency. I used Q solution that comes with hotstar PCR master mix and it gives me a very good amplicon.

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by roniadam
Sat Oct 10, 2009 9:15 am
 
Forum: Molecular Biology
Topic: nested pcr
Replies: 3
Views: 1388

vaccine

... so the culture filtrate can be used as the vaccine after it is precipitated on aluminum hydroxide (a particulate material that also serves as an adjuvant). Disease producing bacteria can be modified by growing them in adverse conditions. In time this may result in living bacteria that can no ...

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by kiekyon
Sat Apr 08, 2006 12:17 pm
 
Forum: Microbiology
Topic: vaccine
Replies: 5
Views: 7207

WESTERN BLOTTING ARTICLES

... on the rest of the gel. Carefully cut out the band and soak it in 1 mL PBS buffer. Crush it and make an emulsion with 1 mL Freund's Complete Adjuvant (which is an oily substance). The complete adjuvant contains microbacteria (an immune stimulant) to increase the immune response. Inject this ...

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by Dr.Stein
Mon Nov 28, 2005 9:02 am
 
Forum: Molecular Biology
Topic: WESTERN BLOTTING ARTICLES
Replies: 3
Views: 4289


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