Dictionary » A » Adherent

Adherent

Adherent

1. Sticking; clinging; adhering.

2. Attached as an attribute or circumstance.

3. (Science: botany) Congenitally united with an organ of another kind, as calyx with ovary, or stamens with petals.

Origin: L. Adhaerens, -entis, p. Pr., cf. F. Adherent.


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peripheral mononuclear cells handling

... incubation of 24h I would like to monitor the presence of a certain protein on their surface using a labelled antibody. Do I need to treat them as adherent cells using trypsin before adding the mix with the antibody or can I resuspend them pipetting? What do yo think? thanks a lot!

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by claire84
Thu Nov 01, 2012 12:18 pm
 
Forum: Cell Biology
Topic: peripheral mononuclear cells handling
Replies: 3
Views: 2026

hybridomas

... the hybridomas and that its normal to be able to see the white dots in the culture by just looking at it with your eyes. I'm use to working with adherent cells and suspensions cells so typically the cells aren't as visible unless you use a microscope. I just want to make sure that there is no ...

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by xbritoe
Mon Feb 06, 2012 5:26 am
 
Forum: Cell Biology
Topic: hybridomas
Replies: 1
Views: 1319

Learning tissue culture, specifically moDC generation

... (e.g. 24-well plate) in AIM-V, incubate for, say, 2 hours at +37C and wash the wells "firmly but gently" several times to remove non-adherent cells (i.e. lymphocytes). Add fresh medium and something like 100 ng/ml GM-CSF and incubate for 24 hours. Then add 25 ug/ml of poly-I:C and ...

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by biohazard
Fri Feb 03, 2012 2:09 pm
 
Forum: Cell Biology
Topic: Learning tissue culture, specifically moDC generation
Replies: 2
Views: 2213

MTT at early timepoints after cell seeding

Hi, I´m new to this field. Is it a good idea to quantify amount of adherent cells on titanium at early time-points(30 min, 1h ...) with MTT assay? One thought I had was that newly trypsinized cells that are seeded on a surface are not very happy, which perhaps ...

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by outremer
Tue Jan 25, 2011 12:17 pm
 
Forum: Cell Biology
Topic: MTT at early timepoints after cell seeding
Replies: 0
Views: 724

Re: cell cloning by limiting dilution

... on which cell line you grow, you can obtain easily or difficult a colony from a single cell. You don't tell if cell line you want to clone is adherent or it grows in suspension. Show me your e-mail adress and I'll send you a standard protocol. . Dear Friend, As I am awair that you have very ...

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by anubt22
Mon Sep 06, 2010 5:02 am
 
Forum: Cell Biology
Topic: cell cloning by limiting dilution
Replies: 4
Views: 9150
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